CAJ | 학술논문

为了研究脱氮硫杆菌(Thiobacillus denitrificans, TDN)对硫酸盐还原菌(sulfate-reducing bacteria, SRB)生长的影响,将从污水中分离到的硫酸盐还原菌和从pH为2~3的酸性土壤中分离到的脱氮硫杆菌(TDN)用于含适当浓度硫酸盐和硝酸盐的模拟污水的处理,并测定单菌或混菌培养后系统中硫酸盐、硝酸盐浓度的变化以及硫化氢的产量.结果表明,在仅接种硫酸盐还原菌的培养系统中,硫酸盐和硝酸盐的含量分别降低4.8%和1.0%;而在同时接种脱氮硫杆菌和硫酸盐还原菌的系统中硫酸盐的含量升高了4.7%,但硝酸盐氮含量降低了25%,这一作用随着培养基中硝酸盐起始浓度的提高而得到加强.另外,混菌培养系统的硫化氢浓度比单一硫酸盐还原菌培养系统降低了65.93%.由此推断,在混菌培养系统中,脱氮硫杆菌通过其反硝化过程产生的代谢产物使硫酸盐还原菌的生长环境条件发生改变,从而抑制其生长并减少了硫化氢的产生.这对预防硫酸盐还原菌带来的不利影响提供了有效的措施.
위료연구탈담류간균(Thiobacillus denitrificans, TDN)대류산염환원균(sulfate-reducing bacteria, SRB)생장적영향,장종오수중분리도적류산염환원균화종pH위2~3적산성토양중분리도적탈담류간균(TDN)용우함괄당농도류산염화초산염적모의오수적처리,병측정단균혹혼균배양후계통중류산염、초산염농도적변화이급류화경적산량.결과표명,재부접충류산염환원균적배양계통중,류산염화초산염적함량분별강저4.8%화1.0%;이재동시접충탈담류간균화류산염환원균적계통중류산염적함량승고료4.7%,단초산염담함량강저료25%,저일작용수착배양기중초산염기시농도적제고이득도가강.령외,혼균배양계통적류화경농도비단일류산염환원균배양계통강저료65.93%.유차추단,재혼균배양계통중,탈담류간균통과기반초화과정산생적대사산물사류산염환원균적생장배경조건발생개변,종이억제기생장병감소료류화경적산생.저대예방류산염환원균대래적불리영향제공료유효적조시.
In this paper, SRB strain screened from sewage and Thiobacillus denitrificans strain screened from acidic soil (pH 2~3) were seeded into simulative wastewater containing suitable concentration of sulfate and ni-trate to evaluate the effect of Thiobacillus denitrificans on the growth of SRB. After incubated, the concentration of sulfate and nitrate, and the yield of hydrogen sulfide were determined. The results showed that in the culture sys-tem with SRB only, the concentration of sulfate and nitrate were decreased by 4.8%and 1%, respectively;howev-er, in the mixed culture system with SRB and TDN, the concentration of sulfate was increased by 4.7%, but the concentration of nitrate was decreased by 25%and the effectiveness was strengthened with the increase of initial concentration of nitrate in medium. In addition, compared with the culture system with SRB only, the concentra-tion of hydrogen sulfide generated in the mixed system was reduced by 65.93%. It inferred that in the mixed sys-tem, those metabolites produced by the denitrification process of TDN may altered the environmental conditions, thus inhibited the growth of SRB and reduced the production of hydrogen sulfide. It provides an effective measure to prevent the adverse effect caused by SRB.