CAJ | 학술논문

拮抗菌通过分泌细胞壁降解酶降解病原菌细胞壁组分是其重要的拮抗方式.本研究以2个拮抗菌(绿色木霉和枯草芽孢杆菌)和7个植物病原菌为研究对象,对碳水化合物酶类CAZymes (Carbohydrate-active enzymes)进行了注释和比较分析,明确了拮抗菌相对于病原菌发生扩增的细胞壁降解酶亚家族;利用ClustalX 2.0多序列比对、Mega4.0构建系统发育树和MEME软件预测保守基序的方法,分析了扩增亚家族的序列结构和进化特征.结果表明,拮抗菌中可能参与病原菌细胞壁降解酶类的亚家族CBM50、GH25和GH73发生了显著扩增,并通过序列比较和进化分析初步明确了扩增的亚家族与拮抗菌特异降解病原菌细胞壁组分之间存在关联,为拮抗菌细胞壁降解酶类亚家族CBM50、GH25和GH73参与拮抗的分子机理提供理论依据.
길항균통과분비세포벽강해매강해병원균세포벽조분시기중요적길항방식.본연구이2개길항균(록색목매화고초아포간균)화7개식물병원균위연구대상,대탄수화합물매류CAZymes (Carbohydrate-active enzymes)진행료주석화비교분석,명학료길항균상대우병원균발생확증적세포벽강해매아가족;이용ClustalX 2.0다서렬비대、Mega4.0구건계통발육수화MEME연건예측보수기서적방법,분석료확증아가족적서렬결구화진화특정.결과표명,길항균중가능삼여병원균세포벽강해매류적아가족CBM50、GH25화GH73발생료현저확증,병통과서렬비교화진화분석초보명학료확증적아가족여길항균특이강해병원균세포벽조분지간존재관련,위길항균세포벽강해매류아가족CBM50、GH25화GH73삼여길항적분자궤리제공이론의거.
The important way of antagonistic mechanism in antagonistic microorganisms is to degrade the pathogen cell wall by secreted cell-wall related enzymes. In this research, the CAZymes of two antagonistic mi-croorganisms and seven pathogens were annotated with CAZymes database, and then identified the expansion CAZymes subfamilies in antagonistic microorganisms compared to pathogens. Moreover, the sequences character-istic and phylogenetic of expansion CAZymes subfamilies were analyzed by ClustalX 2.0, Mega4.0 and MEME softwares. The results showed that the cell wall degradation subfamilies of CBM50, GH25 and GH73 expanded in antagonistic microorganisms compared to pathogens, and these expansion subfamilies were close relatively to the specific degradation of pathogen cell wall. It is suppose that the CBM50, GH25 and GH73 subfamilies of antago-nistic microorganisms play an important role in the antagonistic mechanism to pathogens.