解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2013年
4期
389-391
,共3页
子宫内膜癌%小干扰RNA%组蛋白去甲基化酶
子宮內膜癌%小榦擾RNA%組蛋白去甲基化酶
자궁내막암%소간우RNA%조단백거갑기화매
endometrial cancinoma%SiRNA%JARID1A
目的研究SiRNA干扰组蛋白去甲基化酶(JARID1A)基因表达对人子宫内膜癌Ishikawa细胞增殖凋亡的影响.方法 Ishi-kawa细胞转染JARID1A特异性的SiRNA;将Ishikawa细胞分成sicon组、sicon+e2组、siJARID1A组和siJARID1A+e2组, RT-PCR检测四组细胞孕激素受体(progesterone receptor,PR)mRNA表达,Western Blot检测四组细胞PR蛋白,MTT法观察细胞增殖,流式细胞术检测细胞周期.结果干扰JARID1A能显著上调PR表达,E2可进一步上调PR表达(P<0.01);E2能促进Ishikawa细胞增殖,转染SiRNA 48 h后细胞增殖受到抑制;转染SiRNA抑制细胞G1、G2/M期(P<0.05).结论 JARID1A SiRNA能有效抑制Ishikawa细胞增殖.
目的研究SiRNA榦擾組蛋白去甲基化酶(JARID1A)基因錶達對人子宮內膜癌Ishikawa細胞增殖凋亡的影響.方法 Ishi-kawa細胞轉染JARID1A特異性的SiRNA;將Ishikawa細胞分成sicon組、sicon+e2組、siJARID1A組和siJARID1A+e2組, RT-PCR檢測四組細胞孕激素受體(progesterone receptor,PR)mRNA錶達,Western Blot檢測四組細胞PR蛋白,MTT法觀察細胞增殖,流式細胞術檢測細胞週期.結果榦擾JARID1A能顯著上調PR錶達,E2可進一步上調PR錶達(P<0.01);E2能促進Ishikawa細胞增殖,轉染SiRNA 48 h後細胞增殖受到抑製;轉染SiRNA抑製細胞G1、G2/M期(P<0.05).結論 JARID1A SiRNA能有效抑製Ishikawa細胞增殖.
목적연구SiRNA간우조단백거갑기화매(JARID1A)기인표체대인자궁내막암Ishikawa세포증식조망적영향.방법 Ishi-kawa세포전염JARID1A특이성적SiRNA;장Ishikawa세포분성sicon조、sicon+e2조、siJARID1A조화siJARID1A+e2조, RT-PCR검측사조세포잉격소수체(progesterone receptor,PR)mRNA표체,Western Blot검측사조세포PR단백,MTT법관찰세포증식,류식세포술검측세포주기.결과간우JARID1A능현저상조PR표체,E2가진일보상조PR표체(P<0.01);E2능촉진Ishikawa세포증식,전염SiRNA 48 h후세포증식수도억제;전염SiRNA억제세포G1、G2/M기(P<0.05).결론 JARID1A SiRNA능유효억제Ishikawa세포증식.
Objective To study the interference with siRNA JARID1A gene expression on proliferation and apoptosis of Ishikawa cells in endometrial cancer. Methods Ishikawa cells, transfected with JARID1A siRNA, were divided into sicon group, sicon e2 group, siJARIDIA group, and siJARID1A e2 group. Expressions of progestogen receptor (PR) mRNA and protein in the 4 groups were detected by RT-PCR and Western blot, respectively. Proliferation and apoptosis of Ishikawa cells were assayed by MTT and flow cytometry, respectively. Results Interference with JARID1A could significantly up-regulate the PR expression. E2 could further up-regulate the PR expression and promote the proliferation of Ishikawa cells (P<0.01). The proliferation of Ishikawa cells was inhibited at G1 and G2/M phase 48 h after transfected with siRNA (P<0.05). Conclusion JARID1A siRNA can effectively inhibit the proliferation and apoptosis of Ishikawa cells.
