解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2013年
4期
399-401
,共3页
高尿酸血症%动物模型%复方丹参滴丸%内皮保护
高尿痠血癥%動物模型%複方丹參滴汍%內皮保護
고뇨산혈증%동물모형%복방단삼적환%내피보호
hyperuricemia%animal model%compound red sage root dripping pill%endothelium protection
目的探讨复方丹参滴丸对高尿酸血症(hyperuricemia,HUA)动物模型血尿酸(uricacid,UA)的降低作用及对血管内皮的保护作用,为临床降UA治疗提供新途径.方法38只SPF级雄性昆明小鼠,按体质量随机分为3组:对照组(A)12只(酵母膏+乙胺丁醇+蒸馏水灌胃);别嘌呤醇组(B)13只(酵母膏+乙胺丁醇+别嘌呤醇灌胃);复方丹参滴丸组(C)13只(酵母膏+乙胺丁醇+复方丹参滴丸灌胃).3周后测定各组血清UA和一氧化氮(NO)水平;分离胸腹主动脉,石蜡包埋、切片后HE染色,并进行免疫组化检测核转录因子-κB(NF-κB)和血管内皮细胞黏附因子-1(VCAM-1)的蛋白表达.结果别嘌呤醇组和丹参滴丸组血清UA水平[(372.71±22.16)μmol/L和(328.65±28.78)μmol/L)]与对照组[(605.67±54.76)μmol/L]相比均有显著下降(P<0.01),血清NO水平[(29.55±1.55)μmol/L和35.11±2.03μmol/L)]与对照组[(20.89±1.70)μmol/L)]相比均升高(P<0.01),复方丹参滴丸组优于别嘌呤醇组(P<0.01).对照组主动脉内膜NF-κB和VCAM-1可见连续阳性表达,其余两组表达不同程度减弱.各组主动脉内膜形态和血管壁结构均未发生改变.结论复方丹参滴丸可降低HUA小鼠血清UA水平,提升血清NO水平,效果均优于别嘌呤醇;同时可抑制HUA小鼠主动脉内膜NF-κB和VCAM-1的表达.
目的探討複方丹參滴汍對高尿痠血癥(hyperuricemia,HUA)動物模型血尿痠(uricacid,UA)的降低作用及對血管內皮的保護作用,為臨床降UA治療提供新途徑.方法38隻SPF級雄性昆明小鼠,按體質量隨機分為3組:對照組(A)12隻(酵母膏+乙胺丁醇+蒸餾水灌胃);彆嘌呤醇組(B)13隻(酵母膏+乙胺丁醇+彆嘌呤醇灌胃);複方丹參滴汍組(C)13隻(酵母膏+乙胺丁醇+複方丹參滴汍灌胃).3週後測定各組血清UA和一氧化氮(NO)水平;分離胸腹主動脈,石蠟包埋、切片後HE染色,併進行免疫組化檢測覈轉錄因子-κB(NF-κB)和血管內皮細胞黏附因子-1(VCAM-1)的蛋白錶達.結果彆嘌呤醇組和丹參滴汍組血清UA水平[(372.71±22.16)μmol/L和(328.65±28.78)μmol/L)]與對照組[(605.67±54.76)μmol/L]相比均有顯著下降(P<0.01),血清NO水平[(29.55±1.55)μmol/L和35.11±2.03μmol/L)]與對照組[(20.89±1.70)μmol/L)]相比均升高(P<0.01),複方丹參滴汍組優于彆嘌呤醇組(P<0.01).對照組主動脈內膜NF-κB和VCAM-1可見連續暘性錶達,其餘兩組錶達不同程度減弱.各組主動脈內膜形態和血管壁結構均未髮生改變.結論複方丹參滴汍可降低HUA小鼠血清UA水平,提升血清NO水平,效果均優于彆嘌呤醇;同時可抑製HUA小鼠主動脈內膜NF-κB和VCAM-1的錶達.
목적탐토복방단삼적환대고뇨산혈증(hyperuricemia,HUA)동물모형혈뇨산(uricacid,UA)적강저작용급대혈관내피적보호작용,위림상강UA치료제공신도경.방법38지SPF급웅성곤명소서,안체질량수궤분위3조:대조조(A)12지(효모고+을알정순+증류수관위);별표령순조(B)13지(효모고+을알정순+별표령순관위);복방단삼적환조(C)13지(효모고+을알정순+복방단삼적환관위).3주후측정각조혈청UA화일양화담(NO)수평;분리흉복주동맥,석사포매、절편후HE염색,병진행면역조화검측핵전록인자-κB(NF-κB)화혈관내피세포점부인자-1(VCAM-1)적단백표체.결과별표령순조화단삼적환조혈청UA수평[(372.71±22.16)μmol/L화(328.65±28.78)μmol/L)]여대조조[(605.67±54.76)μmol/L]상비균유현저하강(P<0.01),혈청NO수평[(29.55±1.55)μmol/L화35.11±2.03μmol/L)]여대조조[(20.89±1.70)μmol/L)]상비균승고(P<0.01),복방단삼적환조우우별표령순조(P<0.01).대조조주동맥내막NF-κB화VCAM-1가견련속양성표체,기여량조표체불동정도감약.각조주동맥내막형태화혈관벽결구균미발생개변.결론복방단삼적환가강저HUA소서혈청UA수평,제승혈청NO수평,효과균우우별표령순;동시가억제HUA소서주동맥내막NF-κB화VCAM-1적표체.
Objective To provide a new therapy for reducing uric acid (UA) by studying the role of compound red sage root dripping pill in reducing UA and in protecting endothelium in a hyperuricemia animal model. Methods Thirty-eight SPF-class male Kunming mice were randomly divided into control group (group A, n=12), allopurinol treatment group (group B, n=13), and compound red sage root dripping pill treatment group (group C, n=13). Three weeks after the animals in 3 groups were treated with yeast+ethambutol+distilled water gastric lavage, allopurinol+ethambutol+allopurinol gastric lavage, and yeast+ethambutol+ compound red sage root dripping pill gastric lavage, respectively, their serum UA and NO levels were measured. Thoracico-abdominal aorta was isolated and cut into sections which were stained with H&E. Expressions of NF-κB and VCAM-1 were detected with immunohistochemical staining. Results The serum UA level was significantly lower whereas the serum No level was significantly higher in groups B and C than in group A [(372.71±22.16) μmol/L and (328.65±28.78) μmol/L vs (605.67±54.76) μmol/L, P <0.01;(29.55±1.55) μmol/L and (35.11±2.03) μmol/L vs (20.89±1.70) μmol/L, P <0.01)]. The NF-κB and VCAM-1 were strongly expressed in aorta endomembrane of group A but weakly expressed in groups B and C. No significant change occurred in aorta endomembrane morphology and blood vessel wall structure of 3 groups. Conclusion compound red sage root dripping pill can reduce the serum UA level, down-regulate the expression of NF-κB and VCAM-1, and increase the serum NO level in hyperuricemia mice.
