农业工程学报
農業工程學報
농업공정학보
2013年
7期
1-16
,共16页
污染检测%生物技术%环境监测%生态毒理效应%生物标志物%分子诊断技术
汙染檢測%生物技術%環境鑑測%生態毒理效應%生物標誌物%分子診斷技術
오염검측%생물기술%배경감측%생태독리효응%생물표지물%분자진단기술
pollution detection%biotechnology%monitoring%ecotoxicological effect%biomarker%molecular diagnosis
在环境科学领域中,细胞和分子水平的生物标志物由于其特异性和实用性等优点,在近年来已经被广泛认可.传统的分子生物标志物,如超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malonaldehyde, MDA)、8-羟基脱氧鸟苷(8-hydroxydeoxyguanosine,8-OHdG)等,往往只能表征某类胁迫的程度,但却不能解释损伤和响应发生的分子机理,也不能精确地指示环境污染物的种类.新技术(高效液相色谱,气质联用,酶联免疫法等)的发展提升了传统分子生物标志物的检测灵敏度和特异性.为了加深对分子毒理学机理,筛选和确定新的分子生物标志物,一些新技术常着眼于提高筛选通量,提升可操作性和降低成本(如组学技术,单细胞凝胶电泳,报告生物体).分子诊断的概念起初来源于临床诊断,转而被引入到环境科学研究中来,因为环境和人体一样是一个复杂的系统,环境污染可以看作一个复杂系统的疾病.为了更快更有效地筛选合适的生物标志物,需要广泛开展对模式生物的组学研究和致毒分子机制的研究.同时,也应注意研究过程中的标准化(如谨慎选择暴露浓度和时间,代表物种和其特定的生活史阶段).
在環境科學領域中,細胞和分子水平的生物標誌物由于其特異性和實用性等優點,在近年來已經被廣汎認可.傳統的分子生物標誌物,如超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malonaldehyde, MDA)、8-羥基脫氧鳥苷(8-hydroxydeoxyguanosine,8-OHdG)等,往往隻能錶徵某類脅迫的程度,但卻不能解釋損傷和響應髮生的分子機理,也不能精確地指示環境汙染物的種類.新技術(高效液相色譜,氣質聯用,酶聯免疫法等)的髮展提升瞭傳統分子生物標誌物的檢測靈敏度和特異性.為瞭加深對分子毒理學機理,篩選和確定新的分子生物標誌物,一些新技術常著眼于提高篩選通量,提升可操作性和降低成本(如組學技術,單細胞凝膠電泳,報告生物體).分子診斷的概唸起初來源于臨床診斷,轉而被引入到環境科學研究中來,因為環境和人體一樣是一箇複雜的繫統,環境汙染可以看作一箇複雜繫統的疾病.為瞭更快更有效地篩選閤適的生物標誌物,需要廣汎開展對模式生物的組學研究和緻毒分子機製的研究.同時,也應註意研究過程中的標準化(如謹慎選擇暴露濃度和時間,代錶物種和其特定的生活史階段).
재배경과학영역중,세포화분자수평적생물표지물유우기특이성화실용성등우점,재근년래이경피엄범인가.전통적분자생물표지물,여초양화물기화매(superoxide dismutase, SOD)、병이철(malonaldehyde, MDA)、8-간기탈양조감(8-hydroxydeoxyguanosine,8-OHdG)등,왕왕지능표정모류협박적정도,단각불능해석손상화향응발생적분자궤리,야불능정학지지시배경오염물적충류.신기술(고효액상색보,기질련용,매련면역법등)적발전제승료전통분자생물표지물적검측령민도화특이성.위료가심대분자독이학궤리,사선화학정신적분자생물표지물,일사신기술상착안우제고사선통량,제승가조작성화강저성본(여조학기술,단세포응효전영,보고생물체).분자진단적개념기초래원우림상진단,전이피인입도배경과학연구중래,인위배경화인체일양시일개복잡적계통,배경오염가이간작일개복잡계통적질병.위료경쾌경유효지사선합괄적생물표지물,수요엄범개전대모식생물적조학연구화치독분자궤제적연구.동시,야응주의연구과정중적표준화(여근신선택폭로농도화시간,대표물충화기특정적생활사계단).
@@@@Cellular and molecular biomarkers have been widely accepted in these years because of their strength of specific, monitoring and utilitarian. However, conventional biomarkers can only indicate the class of stress, but fail to explain why damage or response happens or, to indicate a specific pollutant. Thus, environmental monitoring and risk assessment are calling for utilitarian biomarkers and their measurement techniques. Recently, several studies have developed new techniques (e.g. high efficiency liquid chromatography and its mass spectrum, gas phase chromatography and its mass spectrum, enzyme-linked immunosorbent assay) for detecting molecular biomarkers, such as superoxide dismutase (SOD), malonaldehyde (MDA), 8-hydroxydeoxyguanosine (8-OhdG), etylcholinesterase (AchE), glutathione (GSH), heat shock proteins (HSPs), metallothionein(MT). Besides, many efforts focus on perfecting the extant techniques for easier operability or lower cost. We believe that techniques which are low-cost, or trace amount detectable or quickly and easily operative will be preferent in new biomarkers screening and validation. The screening, validation, and their evaluation methods need standardization before biomarkers are widely used in realistic environmental monitoring and management, because there is high diversity among different species and different exposure. In our opinion, for a certain pollutant, typical subject organisms in a specific period of its life history should be chosen and well studied the toxic response mechanisms induced by range concentrations and range exposure periods of toxic chemicals. Not only the extant biomarkers’research is in progress, but the new generation techniques, such as DNA chips, comet assay, bioreporter and omics methodology had emerged. They have offered a new sight to understand ecotoxicology, and powerful tools to explore new biomarkers. Omics and DNA chips may help to screen new responding gene specifically sensitive to a class of toxicants or particular one. The screening process is that: firstly prepare cDNA library derived from creatures exposed to pollutants, secondly analyze the differences of the mRNAs (genes) translation profile between blank control and treatments to summarize a specific responding profile for a particular pollutant, thirdly confirm and optimize the respond combination of genes in the field study. Comet assay is a promising and low-cost method to identify genotoxicants in the low level exposure. By using comet tail parameters, comet assay can determine the DNA damage of eukaryotes including human being. The standardized 96-well plate style comet assay kit allows scientists to detect numeric samples, especially for occupational health check. Bioreporters are the genetic modified organism, most of which are bacteria, and can monitoring environment risk outside the laboratory by fluorescence. Molecular biology has provided new strategies to explore and to study biomarkers. The intersection of molecular biology and environmental science can arise more and more promising knowledge and methodologies. The concept of molecular diagnosis was come up in the disease diagnosis area. However, it is also fit for environmental science, because pollutions are like diseases of the Mother Nature. Thus, the research frontiers, such as new strategies and techniques of disease molecular diagnosis are also very valuable for environmental monitoring and management.