上海医药
上海醫藥
상해의약
SHANGHAI MEDICAL & PHARMACEUTICAL JOURNAL
2013年
6期
26-30
,共5页
蔡德培**%乔丽丽%郑力行
蔡德培**%喬麗麗%鄭力行
채덕배**%교려려%정역행
环境内分泌干扰物%儿童性早熟%高效液相色谱
環境內分泌榦擾物%兒童性早熟%高效液相色譜
배경내분비간우물%인동성조숙%고효액상색보
environmental endocrine disruptors%precocious puberty%high performance liquid chromatography
目的:探讨环境内分泌干扰物(EEDs)与儿童性早熟发病的关系.方法:收集2005年3月至2005年9月在性早熟专科门诊就诊的初诊患儿110例及正常儿童100例.采用高效液相色谱及气相色谱分析法测定性早熟患儿及正常儿童血清中辛基酚(OP)、双酚A(BPA)和邻苯二甲酸二丁酯(DBP)的含量.同时测定患儿的子宫、卵巢体积和血清雌二醇(E2)含量.将血清EEDs含量与靶器官发育的指标作相关性分析.结果:正常儿童中,5.0%血清检测到OP(中位数,下同)为2.72 ng/ml,2.0%检测到BPA(2.11 ng/ml),4.0%检测到DBP(0.52 ng/ml);性早熟患儿中,33.6%血清检测到OP(7.38 ng/ml),40.9%检测到BPA(9.15 ng/ml),27.3%检测到DBP(2.76 ng/ml).性早熟患儿血清中EEDs含量显著高于正常儿童(P<0.001).患儿血清OP、BPA、DBP含量与其子宫体积均呈显著正相关(P<0.05或0.01),血清OP、DBP含量与其卵巢体积均呈显著正相关(P<0.05或0.01).结论:当前的正常儿童已有一部分暴露于EEDs,性早熟患儿EEDs的暴露程度比正常儿童严重得多.EEDs与性早熟的发病密切相关,是其重要的致病因素之一.
目的:探討環境內分泌榦擾物(EEDs)與兒童性早熟髮病的關繫.方法:收集2005年3月至2005年9月在性早熟專科門診就診的初診患兒110例及正常兒童100例.採用高效液相色譜及氣相色譜分析法測定性早熟患兒及正常兒童血清中辛基酚(OP)、雙酚A(BPA)和鄰苯二甲痠二丁酯(DBP)的含量.同時測定患兒的子宮、卵巢體積和血清雌二醇(E2)含量.將血清EEDs含量與靶器官髮育的指標作相關性分析.結果:正常兒童中,5.0%血清檢測到OP(中位數,下同)為2.72 ng/ml,2.0%檢測到BPA(2.11 ng/ml),4.0%檢測到DBP(0.52 ng/ml);性早熟患兒中,33.6%血清檢測到OP(7.38 ng/ml),40.9%檢測到BPA(9.15 ng/ml),27.3%檢測到DBP(2.76 ng/ml).性早熟患兒血清中EEDs含量顯著高于正常兒童(P<0.001).患兒血清OP、BPA、DBP含量與其子宮體積均呈顯著正相關(P<0.05或0.01),血清OP、DBP含量與其卵巢體積均呈顯著正相關(P<0.05或0.01).結論:噹前的正常兒童已有一部分暴露于EEDs,性早熟患兒EEDs的暴露程度比正常兒童嚴重得多.EEDs與性早熟的髮病密切相關,是其重要的緻病因素之一.
목적:탐토배경내분비간우물(EEDs)여인동성조숙발병적관계.방법:수집2005년3월지2005년9월재성조숙전과문진취진적초진환인110례급정상인동100례.채용고효액상색보급기상색보분석법측정성조숙환인급정상인동혈청중신기분(OP)、쌍분A(BPA)화린분이갑산이정지(DBP)적함량.동시측정환인적자궁、란소체적화혈청자이순(E2)함량.장혈청EEDs함량여파기관발육적지표작상관성분석.결과:정상인동중,5.0%혈청검측도OP(중위수,하동)위2.72 ng/ml,2.0%검측도BPA(2.11 ng/ml),4.0%검측도DBP(0.52 ng/ml);성조숙환인중,33.6%혈청검측도OP(7.38 ng/ml),40.9%검측도BPA(9.15 ng/ml),27.3%검측도DBP(2.76 ng/ml).성조숙환인혈청중EEDs함량현저고우정상인동(P<0.001).환인혈청OP、BPA、DBP함량여기자궁체적균정현저정상관(P<0.05혹0.01),혈청OP、DBP함량여기란소체적균정현저정상관(P<0.05혹0.01).결론:당전적정상인동이유일부분폭로우EEDs,성조숙환인EEDs적폭로정도비정상인동엄중득다.EEDs여성조숙적발병밀절상관,시기중요적치병인소지일.
@@@@Objective:To explore the relationship of the environmental endocrine disruptors(EEDs) with the onset of precocious puberty. Method:The blood samples were collected from 110 cases of precocious puberty and 100 cases of normal children who initially visited the precocious puberty outpatient department from March to Sept. 2005. The concentrations of octylphenol (OP), bisphenol A (BPA) and di-n-butyl phthalate (DBP) in the serum were measured with high performance liquid chromatography(HPLC) and gas chromatography. The volume of uterus and ovary, and the content of estradiol (E2) in the serum of precocious puberty were determined at the same time. The contents of EEDs in the serums and the indices of the target organs were analyzed by the methods of correlation and regression. Result:In normal control group, OP、BPA、DBP were detected as [2.72 μg/L, 2.11 μg/L, 0.52 μg/L(median, the same below)] in 5%, 2%, 4%of serum samples respectively. In precocious puberty group, OP、BPA、DBP,(7.38 μg/L, 9.15 μg/L, 2.76 μg/L)in 33.6%, 40.9%, 27.3%of serum samples respectively. The levels of EEDs in the serum of the precocious puberty group were notably higher than that of the control group (P<0.001). In precocious puberty group, positive correlations were found between the contents of OP, BPA, DBP and the volume of uterus (P<0.05 or 0.01). The contents of OP, DBP and the volume of ovary also had a positive correlation (P<0.05or0.01). Conclusion:At present part of the normal children has been contaminated by EEDs, and the children of precocious puberty have been much more heavily exposed to EEDs than the normal children. There is a close relationship between EEDs and the onset of precocious puberty, and EEDs are the important factors inducing the disease.
