CAJ | 학술논문

通过差示扫描量热仪(DSC)扫描图谱及核磁共振成像持水性分析,比较100℃加热、500 MPa超高压与未处理生鲜缢蛏的蛋白质变性程度.分别使用枯草杆菌中性蛋白酶、木瓜蛋白酶、胰蛋白酶、胃蛋白酶对缢蛏肉匀浆进行酶解实验,探讨加热和超高压处理对水解率的影响.实验表明:加热更容易使缢蛏蛋白质脱水、变性,超高压处理可以使蛋白质适度变性并维持较高的含水量.对酶解而言,缢蛏蛋白变性程度越大,其水解度越低,生鲜缢蛏原料更容易被蛋白酶水解.
통과차시소묘량열의(DSC)소묘도보급핵자공진성상지수성분석,비교100℃가열、500 MPa초고압여미처리생선액정적단백질변성정도.분별사용고초간균중성단백매、목과단백매、이단백매、위단백매대액정육균장진행매해실험,탐토가열화초고압처리대수해솔적영향.실험표명:가열경용역사액정단백질탈수、변성,초고압처리가이사단백질괄도변성병유지교고적함수량.대매해이언,액정단백변성정도월대,기수해도월저,생선액정원료경용역피단백매수해.
@@@@Sinonovacula constricta lamarck was treated by heating at 100 ℃ for 5 min and high pressure processing at 500 MPa, respectively, then compared the protein denaturation degree with the fresh through the scan map of the differential scanning calorimetric apparatus (DSC) and the water capacity by Magnatic Resonance Imaging (MRI).The homogenate of Sinonovacula constricta lamarck was hydrolyzed bacillus protease, papain, trypsin, and pepsin, respectively. And we studied the influence of heating and hight pressure on the hydrolysis rate. The result showed that heating made its easier to let the protein dehydrated and denatured, while high pressure processing could make proper denaturalization of protein and maintain a higher content. To enzymatic hydrolysis, the greater of the degree of the protein, the lower for the hydrolysis, so the fresh was hydrolysised by protease easier.