食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
1期
47-50
,共4页
赵文越%王雪青*%何晓萍
趙文越%王雪青*%何曉萍
조문월%왕설청*%하효평
钝顶螺旋藻%废水%生长%培养
鈍頂螺鏇藻%廢水%生長%培養
둔정라선조%폐수%생장%배양
Spirulina platensis%wastewater%growth%cultivation
以钝顶螺旋藻(Spirulina platensis)869藻株为实验材料,在不同营养条件下,用纯净水生产废水培养螺旋藻,同时和AB培养基作对照比较.实验结果显示:在每升纯净水生产废水中添加0.5g K2HPO4,8 g NaHCO3、VB 12浓度为0.15μg/L,作为改良废水(1),在改良废水(1)的基础上,再按照AB培养基配方添加As液和PIV液,作为改良废水废水(2),在光强强度4000 lx、温度24.8℃,光暗周期为12 h∶12 h的条件下培养7 d,钝顶螺旋藻液的吸光度值分别能够达到1.713和1.886,此时对应的生物量是1.601 g(DW)/L和1.766 g(DW)/L.同样条件下用AB培养基进行培养,藻液的吸光度为1.802,生物量是1.688 g(DW)/L,显示出用废水培养螺旋藻的可行性.
以鈍頂螺鏇藻(Spirulina platensis)869藻株為實驗材料,在不同營養條件下,用純淨水生產廢水培養螺鏇藻,同時和AB培養基作對照比較.實驗結果顯示:在每升純淨水生產廢水中添加0.5g K2HPO4,8 g NaHCO3、VB 12濃度為0.15μg/L,作為改良廢水(1),在改良廢水(1)的基礎上,再按照AB培養基配方添加As液和PIV液,作為改良廢水廢水(2),在光彊彊度4000 lx、溫度24.8℃,光暗週期為12 h∶12 h的條件下培養7 d,鈍頂螺鏇藻液的吸光度值分彆能夠達到1.713和1.886,此時對應的生物量是1.601 g(DW)/L和1.766 g(DW)/L.同樣條件下用AB培養基進行培養,藻液的吸光度為1.802,生物量是1.688 g(DW)/L,顯示齣用廢水培養螺鏇藻的可行性.
이둔정라선조(Spirulina platensis)869조주위실험재료,재불동영양조건하,용순정수생산폐수배양라선조,동시화AB배양기작대조비교.실험결과현시:재매승순정수생산폐수중첨가0.5g K2HPO4,8 g NaHCO3、VB 12농도위0.15μg/L,작위개량폐수(1),재개량폐수(1)적기출상,재안조AB배양기배방첨가As액화PIV액,작위개량폐수폐수(2),재광강강도4000 lx、온도24.8℃,광암주기위12 h∶12 h적조건하배양7 d,둔정라선조액적흡광도치분별능구체도1.713화1.886,차시대응적생물량시1.601 g(DW)/L화1.766 g(DW)/L.동양조건하용AB배양기진행배양,조액적흡광도위1.802,생물량시1.688 g(DW)/L,현시출용폐수배양라선조적가행성.
@@@@Cultivation Spirulina platensis 869 in the wastewater from production of pure water under different nutrient conditions was studied, for comprehensive utilization of waste water. The experiment results showed that Spirulina platensis 869 cultured in wastewater did not survival, however inoculated in the improved wastewater (1) and (2), growed well, when per liter of the wastewater was modified with 0.5 g K2HPO4,8 g NaHCO3, 0.15μg vitamin B12, as improved wastewater (1), and referring to the formula of AB medium, added As and PIV solution, to the improved wastewater (1), as improved wastewater (2). When the culture conditions of light intensity, temperature, the cycle of light-dark were: 4 000 lx, 24.8 ℃, 12 h ∶ 12 h, respectively, for 7 days, The absorbance value of the Spirulina platensis medium can be achieved, respectively, 1.713 and 1.866, the corresponding biomass is respectively 1.601g (DW)/L and 1.766 g(DW)/L. While the Spirulina Cultured in AB medium under the same conditions, absorbance of the medium is 1.802, the biomass is 1.688 g(DW)/L, which indicating the feasibility of cultivation of Spirulina by wastewater.