CAJ | 학술논문

建立一种利用焦磷酸测序技术检测和鉴定沙门氏菌(Salmonellla)、单核细胞增生李斯特菌(Listeria monocytogenes)、金黄色葡萄球菌(Staphylococcus aureus)和志贺氏菌(Shigella)的方法.通过对4种菌16SrRNA的保守序列可变区的同源性分析,设计出一套适合焦磷酸测序的扩增引物和测序引物,通过可变区位点差异,利用一套通用引物同时鉴别4种食源性致病菌,测序结果与数据库比对判断细菌种属.结果表明:建立的检测方法可在4 h内完成对4种菌的鉴定,测序鉴定结果与国标的传统生化方法完全一致,焦磷酸测序技术可用于食源性致病菌的检测和鉴定.
건립일충이용초린산측서기술검측화감정사문씨균(Salmonellla)、단핵세포증생리사특균(Listeria monocytogenes)、금황색포도구균(Staphylococcus aureus)화지하씨균(Shigella)적방법.통과대4충균16SrRNA적보수서렬가변구적동원성분석,설계출일투괄합초린산측서적확증인물화측서인물,통과가변구위점차이,이용일투통용인물동시감별4충식원성치병균,측서결과여수거고비대판단세균충속.결과표명:건립적검측방법가재4 h내완성대4충균적감정,측서감정결과여국표적전통생화방법완전일치,초린산측서기술가용우식원성치병균적검측화감정.
@@@@The method of pyrosequencing technology for identification of Salmonellla spp, Listeria monocytogenes, Staphylococcus aureus and Shigella cereus was established. According to nucleotide homology analysis of 16S rRNA of 4 food-borne pathogenic bacteria,we designed one set of primer,including one universal PCR primers and one pyrosequencing sequencing primer. The 4 pathogenic bacteria were distinguished on the basis of pyrosequencing signal peaks. The result showed that the identification of four-kinds of food-borne pathogenic bacteria was completed in 4 h, the results of identification of bacteria were consistent with the result of GB/T method. The pyrosequencing method can use for detection and identification of food-borne pathogenic bacteria.