食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
2期
54-56
,共3页
吸光光度法%对乙酰基偶氮羧%大豆%蛋白质
吸光光度法%對乙酰基偶氮羧%大豆%蛋白質
흡광광도법%대을선기우담최%대두%단백질
Spectrophotometry%β-Acetylcarboxyazo%soybean%protein
紫红色的有机试剂对乙酰基偶氮羧在pH2.90的Britton-Robinson缓冲溶液中,能与蛋白质形成蓝色复合物,λmax为669 nm,比试剂本身红移约92 nm,表观摩尔吸光系数ε669 nm=3.09×105 L/(mol·cm),蛋白质在10滋g/mL~100滋g/mL(BSA)范围内遵循比尔定律.0.1%的TrittonX-100存在,吸光度提高23%.探讨了反应的最佳条件,建立了测定蛋白质的光度分析新方法,用于大豆蛋白的测定,方法操作简便,重现性好,基本无干扰.
紫紅色的有機試劑對乙酰基偶氮羧在pH2.90的Britton-Robinson緩遲溶液中,能與蛋白質形成藍色複閤物,λmax為669 nm,比試劑本身紅移約92 nm,錶觀摩爾吸光繫數ε669 nm=3.09×105 L/(mol·cm),蛋白質在10滋g/mL~100滋g/mL(BSA)範圍內遵循比爾定律.0.1%的TrittonX-100存在,吸光度提高23%.探討瞭反應的最佳條件,建立瞭測定蛋白質的光度分析新方法,用于大豆蛋白的測定,方法操作簡便,重現性好,基本無榦擾.
자홍색적유궤시제대을선기우담최재pH2.90적Britton-Robinson완충용액중,능여단백질형성람색복합물,λmax위669 nm,비시제본신홍이약92 nm,표관마이흡광계수ε669 nm=3.09×105 L/(mol·cm),단백질재10자g/mL~100자g/mL(BSA)범위내준순비이정률.0.1%적TrittonX-100존재,흡광도제고23%.탐토료반응적최가조건,건립료측정단백질적광도분석신방법,용우대두단백적측정,방법조작간편,중현성호,기본무간우.
@@@@Purple β-Acetylcarboxyazo could combine with protein form blue complex in Britton-Robinson buffer at pH 2.90, which presents a maximum absorption at 669 nm with 92 nm of red shift compared to β-Acetylcarboxyazoitself.Itsapparentmolarabsorptivityandlinearrangewere3.09×105L/(mol·cm) and 10 μg/mL-100μg/mL (BSA) . Sensitivity increased 23%with the presence of 0.1%of TrittonX-100. A new method for determination of soluble proteins in soybean was established under the optional condition. The method is simple, fast, high sensitivity and good reproducibility.
