CAJ | 학술논문

以低温脱脂大豆粉为原料,对比大豆蛋白分离提取方法,对大豆11S和7S球蛋白提取工艺进行优化研究,并采用SDS-PAGE凝胶电泳测定提取纯度.提取大豆11S和7S球蛋白优化工艺如下:在调节pH到6.4之前,浸提液中添加0.08 mmol/L NaHSO3,5 mmol/L CaCl2,pH6.2提取大豆11S球蛋白.上清液调节pH4.8离心提取大豆7S球蛋白.结果表明,优化工艺对比传统方法有效提高了两种球蛋白提取率和纯度,提取率11S达82.8%,7S达73.2%;提取纯度11S达78.3%;7S达71.1%.
이저온탈지대두분위원료,대비대두단백분리제취방법,대대두11S화7S구단백제취공예진행우화연구,병채용SDS-PAGE응효전영측정제취순도.제취대두11S화7S구단백우화공예여하:재조절pH도6.4지전,침제액중첨가0.08 mmol/L NaHSO3,5 mmol/L CaCl2,pH6.2제취대두11S구단백.상청액조절pH4.8리심제취대두7S구단백.결과표명,우화공예대비전통방법유효제고료량충구단백제취솔화순도,제취솔11S체82.8%,7S체73.2%;제취순도11S체78.3%;7S체71.1%.
@@@@This paper based on low-temperature defatted soybean flour as raw material, Compared with soybean protein separation extraction methods, aiming at optimizing the extraction process of soybean 11S and 7S globulin research, The extraction purity was identified by SDS-PAGE gel electrophoresis. The optimized extraction procedure of soybean 11S and 7S globulin was as follows:before adjust pH value to 6.4,the extract liquid was added 0.08 mmol/L NaHSO3, 5 mmol/L CaCl2, soybean 11S globulin extraction at pH 6.2;liquid supernatant adjust the pH 4.8, extracted soybean 7S globulin. The results showed that the optimization process was better than traditional ones, which provided both globulins with satisfactory protein extraction yields and purities, extraction yields 82.8%for 11S and 73.2%for 7S, extraction purities 78.3%for 11S and 71.1%for 7S respectivly.