食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
6期
85-88
,共4页
RP-HPLC%咖啡豆种仁%咖啡豆果皮%咖啡茎叶%咖啡因
RP-HPLC%咖啡豆種仁%咖啡豆果皮%咖啡莖葉%咖啡因
RP-HPLC%가배두충인%가배두과피%가배경협%가배인
RP-HPLC%coffee seed kernel%coffee pericarp%coffee stems and leaves%caffeine
利用RP-HPLC测定小粒种咖啡不同部位(咖啡豆种仁、果皮和茎叶)中咖啡因含量.色谱柱:Agilent Eclips XDB-C18(Sun,4.6 mm×150 mm);流动相:甲醇-水(30∶70,体积比);流速:1 mL/min;检测波长:280 nm;柱温为30℃;咖啡因标准曲线方程为:Y=11.5662X+2.9827,R2=1.咖啡因在16μg/mL~80μg/mL范围内具有良好的线性关系.测定出攀枝花本地引种的小粒种咖啡豆中咖啡因含量为15.56 g/kg,果皮中含量为13.62 g/kg,茎叶中的含量为21.19 g/kg.精密度实验结果为咖啡豆种仁、果皮和茎叶中含量的RSD分别为1.01%、1.11%、1.59%;重复性实验结果为咖啡豆种仁、果皮和茎叶中咖啡因含量的RSD分别为0.69%、1.79%、1.38%;均小于2%,达到含量测定要求.咖啡豆种仁、果皮和茎叶中咖啡因回收率分别在95.6%~108.4%,94%~103.4%,96.5%~102.6%.
利用RP-HPLC測定小粒種咖啡不同部位(咖啡豆種仁、果皮和莖葉)中咖啡因含量.色譜柱:Agilent Eclips XDB-C18(Sun,4.6 mm×150 mm);流動相:甲醇-水(30∶70,體積比);流速:1 mL/min;檢測波長:280 nm;柱溫為30℃;咖啡因標準麯線方程為:Y=11.5662X+2.9827,R2=1.咖啡因在16μg/mL~80μg/mL範圍內具有良好的線性關繫.測定齣攀枝花本地引種的小粒種咖啡豆中咖啡因含量為15.56 g/kg,果皮中含量為13.62 g/kg,莖葉中的含量為21.19 g/kg.精密度實驗結果為咖啡豆種仁、果皮和莖葉中含量的RSD分彆為1.01%、1.11%、1.59%;重複性實驗結果為咖啡豆種仁、果皮和莖葉中咖啡因含量的RSD分彆為0.69%、1.79%、1.38%;均小于2%,達到含量測定要求.咖啡豆種仁、果皮和莖葉中咖啡因迴收率分彆在95.6%~108.4%,94%~103.4%,96.5%~102.6%.
이용RP-HPLC측정소립충가배불동부위(가배두충인、과피화경협)중가배인함량.색보주:Agilent Eclips XDB-C18(Sun,4.6 mm×150 mm);류동상:갑순-수(30∶70,체적비);류속:1 mL/min;검측파장:280 nm;주온위30℃;가배인표준곡선방정위:Y=11.5662X+2.9827,R2=1.가배인재16μg/mL~80μg/mL범위내구유량호적선성관계.측정출반지화본지인충적소립충가배두중가배인함량위15.56 g/kg,과피중함량위13.62 g/kg,경협중적함량위21.19 g/kg.정밀도실험결과위가배두충인、과피화경협중함량적RSD분별위1.01%、1.11%、1.59%;중복성실험결과위가배두충인、과피화경협중가배인함량적RSD분별위0.69%、1.79%、1.38%;균소우2%,체도함량측정요구.가배두충인、과피화경협중가배인회수솔분별재95.6%~108.4%,94%~103.4%,96.5%~102.6%.
@@@@RP-HPLC determination of different parts of the arabica coffee (coffee seed kernel, peel,stems and leaves) in the caffeine content. Column: Agilent Eclips XDB-C18 (Sun, 4.6 mm ×150 mm);mobile phase:methanol-water (30∶70,V/V);flowrate:1mL/min;detectionwavelength:280nm;columntemperature was30℃;Caffeine standard curve equation:Y=11.566 2X+2.982 7, R2=1. Caffeine in 16μg/mL-80μg/mL range with good linearity. Determination of the local introduction of the Arabica Coffee Panzhihua caffeine content of coffee beans 15.56 g/kg, peel content 13.62 g/kg, stems and leaves of the content of 21.19 g/kg. Precision results for the coffee seeds, peel,stems and leaves content of the RSD were 1.01%, 1.11%, 1.59%;Repeatability results for the coffee seeds,peel,stemsandleaves,respectively,incaffeinecontentofRSD0.69%, 1.79%, 1.38%;were lessthan 2%, achieve assay requirements. Coffee seeds, peel,stemsand leavesof caffeine recoverieswere 95.6%-108.4%,94%-103.4%,96.5%-102.6%.
