CAJ | 학술논문

背景:有研究发现姜黄素预处理、缺血后处理对大鼠肾脏缺血再灌注损伤具有保护作用.目的:建立大鼠肾脏缺血再灌注损伤模型,观察姜黄素预处理、缺血后处理、姜黄素预处理联合缺血后处理对大鼠肾脏缺血再灌注损伤的保护作用.方法:60只 SD 雄性大鼠随机均分为5组,假手术组、肾脏缺血再灌注模型组、姜黄素组、缺血后处理组以及联合处理组.肾脏再灌注24 h 后,取下腔静脉血测定肌酐和尿素氮.肾组织测定超氧化物岐化酶活性和丙二醛含量,并行苏木精-伊红染色观察各组肾组织病理学变化.结果与结论:与假手术组比较,其余各组肌酐、尿素氮均升高(P <0.05).与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组的肌酐、尿素氮值在再灌注24 h 后均下降(P <0.05).与姜黄素组和缺血后处理组比较,联合处理组的肌酐更低(P <0.05).与假手术组比较,缺血再灌注模型组超氧化物歧化酶活性明显降低,丙二醛含量明显升高,差异有显著性意义(P <0.05).与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组的超氧化物歧化酶活性升高,丙二醛含量降低,差异有显著性意义(P <0.05).与姜黄素组和缺血后处理组比较,联合处理组的超氧化物歧化酶活性升高,差异有显著性意义(P <0.05).与假手术组比较,缺血再灌注模型组肾小管损伤明显;与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组肾小管损伤减轻明显,与姜黄素组和缺血后处理组比较,联合处理组损伤更轻.说明示姜黄素预处理和缺血后处理联合应用具有协同作用,可以更好的保护大鼠缺血再灌注损伤肾脏.
배경:유연구발현강황소예처리、결혈후처리대대서신장결혈재관주손상구유보호작용.목적:건립대서신장결혈재관주손상모형,관찰강황소예처리、결혈후처리、강황소예처리연합결혈후처리대대서신장결혈재관주손상적보호작용.방법:60지 SD 웅성대서수궤균분위5조,가수술조、신장결혈재관주모형조、강황소조、결혈후처리조이급연합처리조.신장재관주24 h 후,취하강정맥혈측정기항화뇨소담.신조직측정초양화물기화매활성화병이철함량,병행소목정-이홍염색관찰각조신조직병이학변화.결과여결론:여가수술조비교,기여각조기항、뇨소담균승고(P <0.05).여결혈재관주모형조비교,강황소조、결혈후처리조화연합처리조적기항、뇨소담치재재관주24 h 후균하강(P <0.05).여강황소조화결혈후처리조비교,연합처리조적기항경저(P <0.05).여가수술조비교,결혈재관주모형조초양화물기화매활성명현강저,병이철함량명현승고,차이유현저성의의(P <0.05).여결혈재관주모형조비교,강황소조、결혈후처리조화연합처리조적초양화물기화매활성승고,병이철함량강저,차이유현저성의의(P <0.05).여강황소조화결혈후처리조비교,연합처리조적초양화물기화매활성승고,차이유현저성의의(P <0.05).여가수술조비교,결혈재관주모형조신소관손상명현;여결혈재관주모형조비교,강황소조、결혈후처리조화연합처리조신소관손상감경명현,여강황소조화결혈후처리조비교,연합처리조손상경경.설명시강황소예처리화결혈후처리연합응용구유협동작용,가이경호적보호대서결혈재관주손상신장.
BACKGROUND: The studies have found that curcumin preconditioning and ischemic postconditioning have protective effect on renal ischemia-reperfusion injury. OBJECTIVE: To establish a model of renal ischemia-reperfusion injury and observe the protective effect of curcumin preconditioning, ischemic postconditioning and curcumin preconditioning combined with ischemic postconditioning on the renal ischemia-reperfusion injury of rats. METHODS: Sixty Sprague Dawley rats were randomly divided into five groups, namely sham-operation group, renal ischemia-reperfusion model group, curcumin preconditioning group, ischemic postconditioning group and curcumin preconditioning combined with ischemic postconditioning group (combination group). After reperfusion for 24 hours, the inferior vena venous blood was extracted to determine the values of blood creatinine and urea nitrogen. The superoxide dismutase activity and malondialdehyde value were measured in renal tissue, and hematoxylin-eosin staining was used to observe the changes in renal structure. RESULTS AND CONCLUSION: Compared with sham-operation group, the values of blood creatinine and urea nitrogen in other four groups were significantly increased (P < 0.05). Compared with ischemia-reperfusion model group, the values of blood creatinine and urea nitrogen in the urcumin preconditioning group, ischemic postconditioning group and combination group were significantly decreased after reperfusion for 24 hours (P < 0.05). Compared with urcumin preconditioning group and ischemic postconditioning group, the blood creatinine value of the combination group was significantly lower (P < 0.05). Compared with the sham-operation group, superoxide dismutase activity in the ischemia-reperfusion model group was decreased, while the value of malondialdehyde was increased significantly, the differences were statistical y significant (P < 0.05). Compared with ischemia-reperfusion model group, urcumin preconditioning group, ischemic postconditioning group and combination group had higher superoxide dismutase activity, while the value of malondialdehyde was decreased; the differences were statistical y significant (P < 0.05). Compared with urcumin preconditioning group and ischemic postconditioning group, superoxide dismutase activity of the combination group was significantly increased, and the difference was significant (P < 0.05). Compared with the sham-operation group, the renal tubular injury of ischemia-reperfusion injury model group was obvious; compared with ischemia-reperfusion model group, the urcumin preconditioning group, ischemic postconditioning group and the combination group had the significantly reduced tubular injury, and compared with urcumin preconditioning group and ischemic postconditioning group, the combination group had more significantly reduced tubular injury. Our study suggests that curcumin preconditioning combined with ischemic postconditioning has a synergistic effect; it can better protect the kidney from ischemia-reperfusion injury.