尿液中Zimp7早期诊断前列腺癌的潜在价值
뇨액중Zimp7조기진단전렬선암적잠재개치
The significance of urine-detectable Zimp7 for early diagnosis of prostate cancer
  • 万方数据
    中国癌症杂志 중국암증잡지
    CHINA ONCOLOGY
    2013年 2期 87-92 ,共6页

    曹达龙%孙自捷%万方宁%姚旭东%张世林%戴波%张海梁%沈益君%朱耀%朱一平%施国海%马春光%肖文军%秦晓健%林国文%叶定伟

    조체룡%손자첩%만방저%요욱동%장세림%대파%장해량%침익군%주요%주일평%시국해%마춘광%초문군%진효건%림국문%협정위

    前列腺癌%早期诊断%Zimp7%尿液 前列腺癌%早期診斷%Zimp7%尿液
    전렬선암%조기진단%Zimp7%뇨액
    Prostate Cancer%Early diagnosis%Zimp7%Urine
    背景与目的:雄激素受体(androgen receptor,AR)辅助因子失调将激活AR并直接促进前列腺癌的发生、发展.Zimp7(human zinc finger-containing,Miz1,PIAS-like protein on chromosome 7)是一新发现的AR辅助因子,本研究的前期工作证实,其在中国前列腺癌组织标本中高表达,预示着其可能是前列腺癌的一种特异性标志物.本研究旨在探讨尿液中Zimp7在前列腺癌早期诊断中的应用价值.方法:收集97例患者的尿液标本,其中42例前列腺癌患者,50例前列腺良性病变患者,5例膀胱癌患者.采用实时荧光定量PCR技术(real-time quantitative polymerase chain reaction,RT-PCR)检测Zimp7 mRNA的表达.结果:当前列腺特异抗原(prostate specific antigen,PSA)为4~10 ng/mL时Zimp7的受试者工作特征曲线下面积(AUC)为0.850(P=0.002),Zimp7阈值设定为53.00时其诊断前列腺癌的灵敏度、特异度、阳性预测值、阴性预测值分别为77.8%、84.6%、63.6%和91.7%;当不进行PSA分层时,Zimp7的AUC值为0.684(P=0.004);当阈值设定为17.26时,其灵敏度、特异度、阳性预测值、阴性预测值分别为66.7%、63.0%、60.5%和69.0%.另外,未发现Zimp7与年龄、PSA值和Gleason值之间有显著相关性.结论:检测尿液中Zimp7 mRNA是一种无创的、可行的前列腺癌早期诊断方法.
    배경여목적:웅격소수체(androgen receptor,AR)보조인자실조장격활AR병직접촉진전렬선암적발생、발전.Zimp7(human zinc finger-containing,Miz1,PIAS-like protein on chromosome 7)시일신발현적AR보조인자,본연구적전기공작증실,기재중국전렬선암조직표본중고표체,예시착기가능시전렬선암적일충특이성표지물.본연구지재탐토뇨액중Zimp7재전렬선암조기진단중적응용개치.방법:수집97례환자적뇨액표본,기중42례전렬선암환자,50례전렬선량성병변환자,5례방광암환자.채용실시형광정량PCR기술(real-time quantitative polymerase chain reaction,RT-PCR)검측Zimp7 mRNA적표체.결과:당전렬선특이항원(prostate specific antigen,PSA)위4~10 ng/mL시Zimp7적수시자공작특정곡선하면적(AUC)위0.850(P=0.002),Zimp7역치설정위53.00시기진단전렬선암적령민도、특이도、양성예측치、음성예측치분별위77.8%、84.6%、63.6%화91.7%;당불진행PSA분층시,Zimp7적AUC치위0.684(P=0.004);당역치설정위17.26시,기령민도、특이도、양성예측치、음성예측치분별위66.7%、63.0%、60.5%화69.0%.령외,미발현Zimp7여년령、PSA치화Gleason치지간유현저상관성.결론:검측뇨액중Zimp7 mRNA시일충무창적、가행적전렬선암조기진단방법.
    @@@@Background and purpose:The dys-regulation of androgen receptor (AR) co-factor may activate AR and directly contribute to prostate cancer development and progression. The overexpression of Zimp7 (human zinc finger-containing, Miz1, PIAS-like protein on chromosome 7), which had been verified as a newly discovered AR cofactor, was then detected in Chinese prostate cancer tissue in our polit experiment, suggesting that it may be a specific biomarker for prostate cancer. The purpose of this study was to investigate the value of urine Zimp7 for early diagnosis of prostate cancer. Methods:Ninety seven urine samples from 42 patients with prostate cancer were collected, 50 patients with benign lesion of prostate, 5 patients with bladder cancer were collected. The expression pattern of Zimp7 mRNA in urine was determined using real-time quantitative polymerase chain reaction method. Results:In the subgroup patients, the prostate specific antigen (PSA) level was 4-10 ng/mL, the area under the curve (AUC) of Zimp7 was 0.850 (P=0.002);and when the threshold of Zimp7 set as 53.00, the sensitivity, specificity, positive and negative predictive value of detecting prostate cancer were 77.8%, 84.6%, 63.6%and 91.7%, respectively. In the cohort of all patients, the AUC of Zimp7 was 0.684 (P=0.004);and when the cutoff set as 17.26, the sensitivity, specificity, positive and negative predictive value were 66.7%, 63.0%, 60.5%and 69.0%, respectively. Additionally, non-significant correlation of Zimp7 with age, serum PSA, and biopsy Gleason score were found in both population. Conclusion:Detecting urine Zimp7 mRNA is one of non-invasive, feasible and potential methods for early diagnosis of prostate cancer.
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