CAJ | 학술논문

目的:探讨芬太尼对MCF-7乳腺癌细胞增殖、迁移和凋亡的影响.方法:将MCF-7乳腺癌细胞分为6组,正常对照组(C组),芬太尼组(F1组、F2组、F3组、F4组、F5组),芬太尼的终浓度分别为1、0.1、0.01、0.001、0.0001μmol/L,在含去激素新生小牛血清的无酚红培养基培养条件下,分别采用MTT法、划痕实验、Annexin V-FITC细胞凋亡检测试剂盒结合流式细胞仪观察芬太尼对MCF-7乳腺癌细胞增殖、迁移和凋亡的影响.结果:芬太尼组各组OD值、迁移距离和凋亡率与C组比较差异无统计学意义(P>0.05).结论:在含去激素新生小牛血清的无酚红培养基培养条件下,芬太尼对MCF-7乳腺癌细胞增殖、迁移和凋亡无明显影响.
목적:탐토분태니대MCF-7유선암세포증식、천이화조망적영향.방법:장MCF-7유선암세포분위6조,정상대조조(C조),분태니조(F1조、F2조、F3조、F4조、F5조),분태니적종농도분별위1、0.1、0.01、0.001、0.0001μmol/L,재함거격소신생소우혈청적무분홍배양기배양조건하,분별채용MTT법、화흔실험、Annexin V-FITC세포조망검측시제합결합류식세포의관찰분태니대MCF-7유선암세포증식、천이화조망적영향.결과:분태니조각조OD치、천이거리화조망솔여C조비교차이무통계학의의(P>0.05).결론:재함거격소신생소우혈청적무분홍배양기배양조건하,분태니대MCF-7유선암세포증식、천이화조망무명현영향.
@@@@Objective:To study the effects of fentanyl on the proliferation ,migration and apoptosis of human breast carcinoma cell line MCF-7. Method:The MCF-7 cells were divided into six groups:normal control group(group C),fentanyl groups(group F1,group F2,group F3,group F4,group F5),fentanyl groups were exposed to fentanyl 1,0.1,0.01,0.001,0.0001μmol/L respectively.MCF-7 cells grown in Phenol red-free media containing fetal bovine serum which had been removed of hormones. The effects of fentanyl on the proliferation ,migration and apoptosis of human breast carcinoma cell line MCF-7 were assessed by MTT method,scratch wound assay and Annexin V-FITC apoptosis kit respectively.Result:Compared to the control group,there was no significant difference revealed in the optical density ,migration distance and apoptosis rate.Conclusion:When grown in Phenol red-free media containing fetal bovine serum which had been removed of hormones,there is no significant influences of fentanyl on MCF-7 breast cancer cells,proliferation ,migration and apoptosis rate .