CAJ | 학술논문

目的:观察复方芪苓无糖颗粒对高脂损伤血管内皮细胞的保护作用,探讨其治疗高脂血症的作用机制.方法:用高脂血清造成人血管内皮细胞(EVC)损伤模型,以复方芪苓无糖颗粒及血脂康含药血清进行干预.光镜下观察细胞形态变化,MTT法检测细胞活性;采用逆转录-聚合酶链反应(RT-PCR)技术检测复方芪苓无糖颗粒高、中、低剂量各组对高脂血清诱导的血管内皮细胞表面黏附分子(TGF-β1)基因表达.结果:复方芪苓无糖颗粒各剂量组及血脂康组均能使高脂损伤的EVC形态趋于正常,活性增强,细胞膜损伤减轻;并均有上调内皮细胞TGF-β1的趋势,血脂康组TGF-β1表达与复方芪苓颗粒中、高浓度组比较无统计学意义(P>0.05).结论:复方芪苓无糖颗粒对高脂损伤的血管内皮细胞有保护作用,可能是其防治高脂血症及动脉粥样硬化的作用机制之一.
목적:관찰복방기령무당과립대고지손상혈관내피세포적보호작용,탐토기치료고지혈증적작용궤제.방법:용고지혈청조성인혈관내피세포(EVC)손상모형,이복방기령무당과립급혈지강함약혈청진행간예.광경하관찰세포형태변화,MTT법검측세포활성;채용역전록-취합매련반응(RT-PCR)기술검측복방기령무당과립고、중、저제량각조대고지혈청유도적혈관내피세포표면점부분자(TGF-β1)기인표체.결과:복방기령무당과립각제량조급혈지강조균능사고지손상적EVC형태추우정상,활성증강,세포막손상감경;병균유상조내피세포TGF-β1적추세,혈지강조TGF-β1표체여복방기령과립중、고농도조비교무통계학의의(P>0.05).결론:복방기령무당과립대고지손상적혈관내피세포유보호작용,가능시기방치고지혈증급동맥죽양경화적작용궤제지일.
Objective: To study the protective effect of sugar-free Qiling compound granules on endothelial vascu?lar cells(EVC) against injuries by hyperlipidemic serum, in an attempt to understand the underlying mechanism of sugar-free Qiling compound granules in treating hyperlipidemia. Methods: Hyperlipidemic serum was used to establish injured model of EVC. Sera with sugar-free Qiling compound granules of low, medium, high concentra?tions and Xuezhikang were applied with hyperlipidemic serum together. The morphologic changes of EVC were ob?served under light microscope and EVC activity was determined by MTT method. Reverse transcription-poly?merase chain reaction(RT-PCR) technique was used to detect hyperlipidemia induced EVC surface adhesion mole?cule(TGF-β1) gene expression in 3 Qiling groups. Results: After treatment of 3 doses of Qiling compound and Xuezhikang, abnormal morphologic changes of EVC induced by hyperlipidemic serum gradually retained to nor?mal, the activity of EVC increased, and the injury of cell membrane lessened. The mRNA’s expression of TGF-β1 in all groups increased after treatment and the expression was not statistically different between the Xuezhi?kang group and the sugar-free Qiling groups with medium and high concentrations(P>0.05). Conclusion:Sug?ar-free Qiling compound granules have a protective effect on EVC against injuries induced by hyperlipidemic se?rum and can be used as prevention and treatment for hyperlipidemia and atherosclerosis ideal medication.