浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
4期
262-264
,共3页
银屑病%HaCaT细胞%清热类中药
銀屑病%HaCaT細胞%清熱類中藥
은설병%HaCaT세포%청열류중약
psoriasis%HaCaT cells%Chinese herbs with properties of clearing away heat
目的:观察单味清热类中药大青叶、水牛角、龙胆草、生地、黄芩提取物对HaCaT细胞增殖的影响.方法:用MTT法检测上述单味清热类中药提取物对HaCat细胞的24h抑制率.结果:大青叶、龙胆草、黄芩、生地提取物具有抑制HaCaT细胞增殖作用,且与药物浓度具有一定相关性;水牛角提取物具有促进HaCaT细胞增殖作用,且与药物浓度具有一定相关性,与空白对照组比较差异具有统计学意义(P<0.05).结论:抑制角质形成细胞增殖可能是大青叶、生地、黄芩、龙胆草治疗银屑病的作用机制之一,水牛角治疗银屑病的作用与抑制角质形成细胞增殖无关.
目的:觀察單味清熱類中藥大青葉、水牛角、龍膽草、生地、黃芩提取物對HaCaT細胞增殖的影響.方法:用MTT法檢測上述單味清熱類中藥提取物對HaCat細胞的24h抑製率.結果:大青葉、龍膽草、黃芩、生地提取物具有抑製HaCaT細胞增殖作用,且與藥物濃度具有一定相關性;水牛角提取物具有促進HaCaT細胞增殖作用,且與藥物濃度具有一定相關性,與空白對照組比較差異具有統計學意義(P<0.05).結論:抑製角質形成細胞增殖可能是大青葉、生地、黃芩、龍膽草治療銀屑病的作用機製之一,水牛角治療銀屑病的作用與抑製角質形成細胞增殖無關.
목적:관찰단미청열류중약대청협、수우각、룡담초、생지、황금제취물대HaCaT세포증식적영향.방법:용MTT법검측상술단미청열류중약제취물대HaCat세포적24h억제솔.결과:대청협、룡담초、황금、생지제취물구유억제HaCaT세포증식작용,차여약물농도구유일정상관성;수우각제취물구유촉진HaCaT세포증식작용,차여약물농도구유일정상관성,여공백대조조비교차이구유통계학의의(P<0.05).결론:억제각질형성세포증식가능시대청협、생지、황금、룡담초치료은설병적작용궤제지일,수우각치료은설병적작용여억제각질형성세포증식무관.
Objective: To investigate the influence of folium, buffalo horn, gentian, habitat, and skullcap extracts on the proliferation of HaCaT cells. Methods: The 24h inhibitory rate of HaCaT cell proliferation by the above mentioned Chinese herbs was examined by the MTT method. Results: Folium, gentian, skullcap, and habitat ex?tracts inhibited the proliferation of HaCaT cells in a dose-dependent manner, while buffalo horn extract advanced the proliferation of the cells in a dose-dependent manner, and these results were all significantly different as compared to blank control groups(P<0.05). Conclusion: One of the mechanisms by which folium, buffalo horn, gentian, and skullcap treat psoriasis may be inhibiting the proliferation of malpighian cells, while buffalo horn does not have the same effect when being used to treat psoriasis.
