浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
4期
265-268
,共4页
急性胰腺炎%小鼠%L-精氨酸
急性胰腺炎%小鼠%L-精氨痠
급성이선염%소서%L-정안산
acute pancreatitis%mice%L-arginine
目的:观察不同pH值L-精氨酸腹腔注射诱导急性胰腺炎模型的效果.方法:70只BABL/C小鼠,随机分为pH11组、pH8组和对照组,分别采用pH值为11的20%L-精氨酸溶液、pH值为8的20%L-精氨酸溶液、等体积生理盐水,间隔1h 2次小鼠腹腔内注射(250mg/100g体质量)的方法.在24h和48h分别观测三组小鼠的血清淀粉酶、胰腺病理学评分,并统计死亡率.结果:pH11组、pH8组小鼠在24h和48h的血清淀粉酶水平均较对照组显著升高(P<0.05,P<0.01);PH11组较pH8组24h和48h血清淀粉酶水平均升高更明显(P<0.01);pH8组24h和48h胰腺病理评分均较pH11组显著降低(P<0.05),同时该组相应时间点的小鼠死亡率也较后者显著下降(P<0.05).结论:L-精氨酸溶液的pH值对胰腺损伤程度和动物的死亡率产生很大影响;精氨酸的强碱性对腹腔脏器的损伤是导致模型病变不稳定性的重要原因.
目的:觀察不同pH值L-精氨痠腹腔註射誘導急性胰腺炎模型的效果.方法:70隻BABL/C小鼠,隨機分為pH11組、pH8組和對照組,分彆採用pH值為11的20%L-精氨痠溶液、pH值為8的20%L-精氨痠溶液、等體積生理鹽水,間隔1h 2次小鼠腹腔內註射(250mg/100g體質量)的方法.在24h和48h分彆觀測三組小鼠的血清澱粉酶、胰腺病理學評分,併統計死亡率.結果:pH11組、pH8組小鼠在24h和48h的血清澱粉酶水平均較對照組顯著升高(P<0.05,P<0.01);PH11組較pH8組24h和48h血清澱粉酶水平均升高更明顯(P<0.01);pH8組24h和48h胰腺病理評分均較pH11組顯著降低(P<0.05),同時該組相應時間點的小鼠死亡率也較後者顯著下降(P<0.05).結論:L-精氨痠溶液的pH值對胰腺損傷程度和動物的死亡率產生很大影響;精氨痠的彊堿性對腹腔髒器的損傷是導緻模型病變不穩定性的重要原因.
목적:관찰불동pH치L-정안산복강주사유도급성이선염모형적효과.방법:70지BABL/C소서,수궤분위pH11조、pH8조화대조조,분별채용pH치위11적20%L-정안산용액、pH치위8적20%L-정안산용액、등체적생리염수,간격1h 2차소서복강내주사(250mg/100g체질량)적방법.재24h화48h분별관측삼조소서적혈청정분매、이선병이학평분,병통계사망솔.결과:pH11조、pH8조소서재24h화48h적혈청정분매수평균교대조조현저승고(P<0.05,P<0.01);PH11조교pH8조24h화48h혈청정분매수평균승고경명현(P<0.01);pH8조24h화48h이선병리평분균교pH11조현저강저(P<0.05),동시해조상응시간점적소서사망솔야교후자현저하강(P<0.05).결론:L-정안산용액적pH치대이선손상정도화동물적사망솔산생흔대영향;정안산적강감성대복강장기적손상시도치모형병변불은정성적중요원인.
Objective: To investigate the effect of acute pancreatitis model induced by different pH values of L-arginine. Methods: Seventy BABL/C mice were randomly divided into 3 groups. Two groups were treated with L-arginine solution of pH 11 and pH 8,respectively. L-arginine solutions were intraperitoneally injected 250mg/100g body weight twice at an interval of 1 hour. Another group served as control and was treated with same vol?ume of saline. Serum amylase was determined and pancreas pathological score and mortality rate were calculated in all groups at the time of 24 and 48 hours after injection. Results: Compared with the control group, the se?rum amylase levels were significantly increased in the pH11 group and pH8 group at the time of 24 and 48 hours(P<0.01). Compared with the pH11 group, the serum amylase levels in the pH8 group were significantly de?creased at the time of 24h and 48h(P<0.01), meanwhile, the pancreas pathological score and mortality rate in the pH8 group were also decreased significantly at both time points(P<0.05). Conclusion: The pH value of L-ar?ginine solution has a significant impact on the degree of pancreatic injury and animal mortality. The strong alka?line of L-arginine which does damage to abdominal organs is an important reason for instability of acute pancre?as model.
