作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2013年
1期
21-28
,共8页
付央%苑冬冬%胡文静%蔡彩平%郭旺珍*
付央%苑鼕鼕%鬍文靜%蔡綵平%郭旺珍*
부앙%원동동%호문정%채채평%곽왕진*
染色体片段置换系%分子标记%QTL%TM-1%Sub18%开放花蕾
染色體片段置換繫%分子標記%QTL%TM-1%Sub18%開放花蕾
염색체편단치환계%분자표기%QTL%TM-1%Sub18%개방화뢰
Chromosome segment substitution lines%Molecular marker%QTL%TM-1%Sub18%Opening bud
Sub18是以陆地棉遗传标准系TM-1为背景,含海岛棉3-79第18染色体的置换系材料.本研究以TM-1为受体亲本,置换系 Sub18为供体亲本,借助分子标记辅助选择技术培育了一套以TM-1为背景,含海岛棉3-79第18染色体不同长度片段的置换系.这套置换系由45个株系构成,共78个置换片段.其中27个株系导入单片段,占总株系的60%;9个株系导入2个片段,占20%;9个株系导入3个及以上片段,占20%.导入片段总长度为467.6 cM,约为该染色体遗传长度的4倍,每个株系内被替换的染色体片段长度不完全相同,平均遗传长度为5.99 cM,最短的为0.9 cM,最长的20.35 cM.其中13个株系表现开放花蕾性状,涉及的最短导入片段长5.05 cM.对TM-1、Sub18以及培育的45个导入系进行农艺性状调查和 QTL 联合定位分析,鉴定出纤维强度(qFS-C18-1)、整齐度(qFU-C18-1)、马克隆值(qFMi-C18-1)、成熟度(qFMa-C18-1)、皮棉重(qLW-C18-1)、籽指(qSI-C18-1)和衣分(qLP-C18-1)7个加性QTL和5个上位性效应QTL.研究结果为进一步精细定位目标QTL、克隆QTL以及重要性状分子设计育种奠定了基础.
Sub18是以陸地棉遺傳標準繫TM-1為揹景,含海島棉3-79第18染色體的置換繫材料.本研究以TM-1為受體親本,置換繫 Sub18為供體親本,藉助分子標記輔助選擇技術培育瞭一套以TM-1為揹景,含海島棉3-79第18染色體不同長度片段的置換繫.這套置換繫由45箇株繫構成,共78箇置換片段.其中27箇株繫導入單片段,佔總株繫的60%;9箇株繫導入2箇片段,佔20%;9箇株繫導入3箇及以上片段,佔20%.導入片段總長度為467.6 cM,約為該染色體遺傳長度的4倍,每箇株繫內被替換的染色體片段長度不完全相同,平均遺傳長度為5.99 cM,最短的為0.9 cM,最長的20.35 cM.其中13箇株繫錶現開放花蕾性狀,涉及的最短導入片段長5.05 cM.對TM-1、Sub18以及培育的45箇導入繫進行農藝性狀調查和 QTL 聯閤定位分析,鑒定齣纖維彊度(qFS-C18-1)、整齊度(qFU-C18-1)、馬剋隆值(qFMi-C18-1)、成熟度(qFMa-C18-1)、皮棉重(qLW-C18-1)、籽指(qSI-C18-1)和衣分(qLP-C18-1)7箇加性QTL和5箇上位性效應QTL.研究結果為進一步精細定位目標QTL、剋隆QTL以及重要性狀分子設計育種奠定瞭基礎.
Sub18시이륙지면유전표준계TM-1위배경,함해도면3-79제18염색체적치환계재료.본연구이TM-1위수체친본,치환계 Sub18위공체친본,차조분자표기보조선택기술배육료일투이TM-1위배경,함해도면3-79제18염색체불동장도편단적치환계.저투치환계유45개주계구성,공78개치환편단.기중27개주계도입단편단,점총주계적60%;9개주계도입2개편단,점20%;9개주계도입3개급이상편단,점20%.도입편단총장도위467.6 cM,약위해염색체유전장도적4배,매개주계내피체환적염색체편단장도불완전상동,평균유전장도위5.99 cM,최단적위0.9 cM,최장적20.35 cM.기중13개주계표현개방화뢰성상,섭급적최단도입편단장5.05 cM.대TM-1、Sub18이급배육적45개도입계진행농예성상조사화 QTL 연합정위분석,감정출섬유강도(qFS-C18-1)、정제도(qFU-C18-1)、마극륭치(qFMi-C18-1)、성숙도(qFMa-C18-1)、피면중(qLW-C18-1)、자지(qSI-C18-1)화의분(qLP-C18-1)7개가성QTL화5개상위성효응QTL.연구결과위진일보정세정위목표QTL、극륭QTL이급중요성상분자설계육충전정료기출.
Substitution line 18 (Sub18) has the background of genetic standard line of G. hirsutum acc. TM-1 with the 18th chro-mosome of G. barbadense accession 3-79. In the study, a set of G. barbadense accession 3-79 chromosome 18 segment substitu-tion lines (CSSL18) were developed via molecular marker-assisted selection (MAS), with TM-1 as a recipient parent and Sub18 as a donor. The developed CSSLs consisted of 45 lines and 78 introgressed segments in total. Of them, 27 were introgressed with single segment, accounting for 60%of the total lines;9 were introgressed with two segments, accounting for 20%;and 9 were introgressed with three or more segments, accounting for 20%. The total length of introgression segments was 467.6 cM, which is 4 times of the genetic length of the chromosome 18. The substituted segment length varied in each line, ranging from the shortest of 0.9 cM to the longest of 20.35 cM, with an average length of 5.99 cM. Further, the opening bud trait was detected in 13 substi-tution lines, with the shortest introgressed segment of 5.05 cM. Using TM-1, Sub18 and 45 substituted lines as materials, we in-vestigated agronomic traits and carried out QTL tagging by joint positioning analysis. Seven additive effect QTLs for fiber strength (qFS-C18-1), uniformity (qFU-C18-1), micronaire (qFMi-C18-1), maturity (qFMa-C18-1), lint weight (qLW-C18-1), seed index (qSI-C18-1), and lint percentage (qLP-C18-1) and five epistatic effect QTLs were identified, respectively. The results will lay a foundation for the fine mapping and cloning of target QTLs, and the molecular breeding by design for pyramiding multi-traits in cotton.
