CAJ | 학술논문

以春性恢复系与半冬性品种(系)杂交后选育的16份新春性恢复系及其4个亲本系[2个半冬性甘蓝型油菜品种(系)和2个春性甘蓝型恢复系]、2个春性甘蓝型不育系为材料,利用SSR、SRAP和AFLP分子标记技术分析材料间的遗传差异,同时利用以上2个春性不育系分别与12个新春性恢复系和1个春性亲本恢复系Ag-5进行NCII双列杂交,测定其杂种优势及杂种表现.16份新恢复系中除931和帐23外,其余的14份新恢复系与2个不育系的遗传距离均大于其春性亲本恢复系与相应不育系的遗传距离,说明导入半冬性品种遗传成分能扩大春性恢复系与不育系间的遗传差异;配制的26个杂交组合中,其双亲中不育系所对应保持系单株产量为高亲值的组合有15个,其中13个组合单株产量超亲优势都强于所对应不育系与亲本恢复系Ag-5所配杂交组合,说明导入半冬性品种遗传成分可增强甘蓝型春油菜杂种优势;12个新恢复系分别与2个不育系所配24个组合中18个组合的单株产量都分别超过所对应不育系与亲本恢复系Ag-5所配杂交组合,说明导入半冬性品种遗传成分能提高甘蓝型春油菜杂种的产量;新恢复系与2个不育系杂交后代的抗病性均强于其亲本恢复系与相应不育系杂交后代的抗病性,说明导入半冬性品种遗传成分能提高春性甘蓝型油菜杂交种抗菌核病的能力.研究结果表明,半冬性甘蓝型油菜品种可为春油菜杂交育种提供有价值的遗传资源.
이춘성회복계여반동성품충(계)잡교후선육적16빈신춘성회복계급기4개친본계[2개반동성감람형유채품충(계)화2개춘성감람형회복계]、2개춘성감람형불육계위재료,이용SSR、SRAP화AFLP분자표기기술분석재료간적유전차이,동시이용이상2개춘성불육계분별여12개신춘성회복계화1개춘성친본회복계Ag-5진행NCII쌍렬잡교,측정기잡충우세급잡충표현.16빈신회복계중제931화장23외,기여적14빈신회복계여2개불육계적유전거리균대우기춘성친본회복계여상응불육계적유전거리,설명도입반동성품충유전성분능확대춘성회복계여불육계간적유전차이;배제적26개잡교조합중,기쌍친중불육계소대응보지계단주산량위고친치적조합유15개,기중13개조합단주산량초친우세도강우소대응불육계여친본회복계Ag-5소배잡교조합,설명도입반동성품충유전성분가증강감람형춘유채잡충우세;12개신회복계분별여2개불육계소배24개조합중18개조합적단주산량도분별초과소대응불육계여친본회복계Ag-5소배잡교조합,설명도입반동성품충유전성분능제고감람형춘유채잡충적산량;신회복계여2개불육계잡교후대적항병성균강우기친본회복계여상응불육계잡교후대적항병성,설명도입반동성품충유전성분능제고춘성감람형유채잡교충항균핵병적능력.연구결과표명,반동성감람형유채품충가위춘유채잡교육충제공유개치적유전자원.
Several B. napus varieties (lines) including two semi-winter rapeseed varieties, two spring restorer lines, two spring male-sterile lines and 16 spring restorer lines (derived from the spring restorer lines and semi-winter rapeseed varieties) were ana-lyzed using SSR, SRAP, and AFLP. Twenty-six combinations were produced according to the North Carolina mating design (NCII) by hand-pollinating 12 new restorer lines and one parental restorer line (Ag-5) with two spring male-sterile lines. The hy-brid performance values were also determined. Among the 16 restorer lines, except for 931 and Zhang 23, the genetic distances were greater between the new restorer lines and two male-sterile lines than between the corresponding parental restorer line (Ag-5) and the two male-sterile lines, showing that introgressing semi-winter varieties into spring restorer lines could increase the genetic distance between spring restorer lines and spring male-sterile lines. The yield per plant for the maintainer lines of 15 combinations, which corresponded to the sterile lines showed high-parent values in 26 combinations, and 13 combinations showed stronger high-parent heterosis of yield per plant compared to combinations produced by the corresponding male-sterile and parental re-storer lines (CMSL×Ag-5), suggesting that introgressing semi-winter varieties into spring restorer lines could enhance the hetero-sis of spring B. napus varieties. Eighteen hybrids among the 24 combinations showed higher yield per plant compared to the com-binations of CMSL×Ag-5, indicating introgressing semi-winter varieties into spring restorer lines might improve the spring B. napus hybrids yield. The results also showed that introgressing semi-winter varieties into spring restorer lines could improve the resistance to Sclerotinia sclerotiorum of spring B. napus hybrids. This study indicates that semi-winter B. napus rapeseed may be a