CAJ | 학술논문

以Bt基因来源于美国和我国的常规棉花品种DP410B和泗抗1号、杂交种岱杂1号和泗抗3号为材料,应用盆栽试验,探讨蕾期18℃低温及不同相对湿度不同时间胁迫对叶片 Bt 杀虫蛋白表达量的影响.结果表明,蕾期18℃下不同湿度6 h胁迫对叶片Bt蛋白的含量都没有显著影响,但胁迫48 h导致2个不同Bt来源常规品种的叶片Bt杀虫蛋白表达量显著下降,与对照相比,下降6.8%~7.2%,杂交种泗抗3号也明显下降,岱杂1号则未受影响;18℃下高湿度与低湿度胁迫间没有显著影响.因此蕾期长时低温会对Bt棉抗虫性有一定影响,影响程度与品种及类型密切相关.
이Bt기인래원우미국화아국적상규면화품충DP410B화사항1호、잡교충대잡1호화사항3호위재료,응용분재시험,탐토뢰기18℃저온급불동상대습도불동시간협박대협편 Bt 살충단백표체량적영향.결과표명,뢰기18℃하불동습도6 h협박대협편Bt단백적함량도몰유현저영향,단협박48 h도치2개불동Bt래원상규품충적협편Bt살충단백표체량현저하강,여대조상비,하강6.8%~7.2%,잡교충사항3호야명현하강,대잡1호칙미수영향;18℃하고습도여저습도협박간몰유현저영향.인차뢰기장시저온회대Bt면항충성유일정영향,영향정도여품충급류형밀절상관.
The objective of this study was to investigate the effect of stresses of 18°C and different relative humidities on the leaf resistance to the insect at squaring stage in Bt cotton. The two conventional cultivars (DP410B and Sikang 1) and two hybrids (Daiza 1 and Sikang 3) from the US and China, respectively, were used. The potted cotton plants were stressed at 18°C and dif-ferent relative humidities (90%, 70%, and 50%) for 6 hours and 48 hours, respectively, then we determined leaf Bt protein con-tents. The results showed that the short period (6 hours) stress of 18°C and different relative humidities obviously had no effect on the leaf Bt protein contents. but the leaf Bt protein contents sharply reduced in the two Bt conventional cultivars after the longer period (48 hours) stress of 18°C and different relative humidities compared with the control, with the reduction from 6.8%to 7.2%. The leaf Bt protein content of hybrid Sikang 3 markedly decreased while that of hybrid Daiza 1 remained unchanged. There was no difference in the effect between the high relative humidity and low relative humidity at 18°C, so the low temperature duration may affect the Bt cotton resistance to insect at squaring stage, which is closely related to cultivars.