作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2013年
2期
191-197
,共7页
普通小麦%大赖草%易位系%分子细胞遗传学%赤霉病抗性
普通小麥%大賴草%易位繫%分子細胞遺傳學%赤黴病抗性
보통소맥%대뢰초%역위계%분자세포유전학%적매병항성
Triticum aestivum%Leymus racemosus%Translocation line%Molecular and cytogenetics%Scab resistance
大赖草7Lr#1S染色体上携带赤霉病抗性基因,将其导入普通小麦有助于增加赤霉病抗源多样性和选育抗赤霉病品种.利用染色体 C-分带、荧光原位杂交和分子标记技术从普通小麦–大赖草7Lr#1单体异附加系的花粉辐射后代中,选育出易位系T7BS·7Lr#1S (NAU639)和T2AS·2AL-7Lr#1S (NAU640).经连续3年大田、温室赤霉病接种鉴定,这2个易位系的赤霉病抗性均显著高于感病亲本中国春、感病对照绵阳8545和石麦4185,为赤霉病抗病育种提供了新的种质资源.
大賴草7Lr#1S染色體上攜帶赤黴病抗性基因,將其導入普通小麥有助于增加赤黴病抗源多樣性和選育抗赤黴病品種.利用染色體 C-分帶、熒光原位雜交和分子標記技術從普通小麥–大賴草7Lr#1單體異附加繫的花粉輻射後代中,選育齣易位繫T7BS·7Lr#1S (NAU639)和T2AS·2AL-7Lr#1S (NAU640).經連續3年大田、溫室赤黴病接種鑒定,這2箇易位繫的赤黴病抗性均顯著高于感病親本中國春、感病對照綿暘8545和石麥4185,為赤黴病抗病育種提供瞭新的種質資源.
대뢰초7Lr#1S염색체상휴대적매병항성기인,장기도입보통소맥유조우증가적매병항원다양성화선육항적매병품충.이용염색체 C-분대、형광원위잡교화분자표기기술종보통소맥–대뢰초7Lr#1단체이부가계적화분복사후대중,선육출역위계T7BS·7Lr#1S (NAU639)화T2AS·2AL-7Lr#1S (NAU640).경련속3년대전、온실적매병접충감정,저2개역위계적적매병항성균현저고우감병친본중국춘、감병대조면양8545화석맥4185,위적매병항병육충제공료신적충질자원.
A resistance gene against wheat scab is located on chromosome 7Lr#1S of Leymus racemosus, which is propitious to enriching scab resistance resources and resistant breeding in wheat (Triticum aestivum L.). The translocation lines NAU639 (T7BS·7Lr#1S) and NAU640 (T2AS·2AL-7Lr#1S) were selected from the plant progenies of irradiated pollens of monosomic alien addition line MA7Lr#1 and confirmed through chromosome FISH-GISH, C-banding, and molecular markers analyses. The two translocation lines had higher resistance to wheat scab than the susceptible parent “Chinese Spring” and the susceptible controls “Mianyang 8545” and “Shimai 4185” in a successive three-year test both in the greenhouse and field. These results showed the stable scab resistance in translocation lines NAU639 and NAU640 and feasibility to be used as a new source in wheat breeding resistant to scab disease.