CAJ | 학술논문

DREB1/CBF类转录因子在植物抵抗外界胁迫上起重要作用,利用这些基因改良作物抗逆性具有重要意义.本研究在白菜中分离到一个DREB类转录因子基因BpDREB1(GenBank登录号为EF219470).该基因序列全长647 bp,推测编码蛋白含213个氨基酸,相对分子量为23 kD,理论等电点为5.11,与白菜中该类转录因子序列同源性为94%.进化树表明, BpDREB1属于DREB亚家族中A1亚族.基因的诱导表达模式分析显示, BpDREB1被低温强烈、迅速诱导表达,并对干旱胁迫也有一定程度的响应,但对高盐处理几乎没有响应.过表达BpDREB1的转基因拟南芥经低温诱导后,其体内可溶性糖及脯氨酸含量大幅度提高.以上结果显示BpDREB1转录因子基因具有家族成员基因结构的特征,在低温、干旱应答途径中起重要作用.
DREB1/CBF류전록인자재식물저항외계협박상기중요작용,이용저사기인개량작물항역성구유중요의의.본연구재백채중분리도일개DREB류전록인자기인BpDREB1(GenBank등록호위EF219470).해기인서렬전장647 bp,추측편마단백함213개안기산,상대분자량위23 kD,이론등전점위5.11,여백채중해류전록인자서렬동원성위94%.진화수표명, BpDREB1속우DREB아가족중A1아족.기인적유도표체모식분석현시, BpDREB1피저온강렬、신속유도표체,병대간한협박야유일정정도적향응,단대고염처리궤호몰유향응.과표체BpDREB1적전기인의남개경저온유도후,기체내가용성당급포안산함량대폭도제고.이상결과현시BpDREB1전록인자기인구유가족성원기인결구적특정,재저온、간한응답도경중기중요작용.
DREB1/CBF transcription factors play an important role in plant stress tolerance, and one of important significance to gain stress-tolerant crops by transgenic technologies. In this study, a DREB-like gene, named BpDREB1 (GenBank accession No. EF219470), was cloned from Chinese cabbage. The BpDREB1 cDNA was 647 bp in length, and encoded protein of 213 amino acids with an predicted molecular weight of 23 kD and a isoelectric point of 5.11, and shared 94%similarity with other DREB transcription factor from Chinese cabbage. On the basis of multiple sequence alignment and phylogenetic analysis, BpDREB1 was classified in A-1 group of the DREB family. The expression patterns analysis indicated that BpDREB1 was strongly up-regulated at low temperature, also responded to dehydration. However, the expression of BpDREB1 was not affected by high salinity. The expression pattern of BpDREB1 was the same as that of other DREB transcription factors in A-1 group. Overexpression of BpDREB1 greatly increased the contents of total soluable sugar and free proline in transgenic Arabidopsis plants, demonstrating that transgenic Arabidopsis induced the expression of soluable sugar and proline related genes to enhance the tolerance to stress. These results suggest that BpDREB1 from Chinese cabbage has the typical characteristic of DREB transcription factors, and func-tions under the stress conditions including low temperature and drought.