CAJ | 학술논문

以草鱼幼鱼为研究对象,用半致死浓度的嗜水气单胞菌感染鱼体,然后取病鱼和对照组的肝、脾、肾经Bouin’s液固定,石蜡包埋、切片、H.E染色,Nikon显微镜观察照相.结果表明:经过感染的鱼体肝、脾、肾都出现不同程度的肿大,肝脏灰白色,脾脏暗紫色.切片观察发现肝脏实质结构破坏明显,肝血管内血细胞减少,肝细胞肿胀、浑浊,空泡化;肾脏间质比例增大,肾小管和肾小体数量明显减少,肾小管上皮细胞肿胀、变性;脾脏内大量充血,脾脏淋巴细胞坏死,H.E染色发现大量的棕色“小结”充斥于整个脾脏之中.
이초어유어위연구대상,용반치사농도적기수기단포균감염어체,연후취병어화대조조적간、비、신경Bouin’s액고정,석사포매、절편、H.E염색,Nikon현미경관찰조상.결과표명:경과감염적어체간、비、신도출현불동정도적종대,간장회백색,비장암자색.절편관찰발현간장실질결구파배명현,간혈관내혈세포감소,간세포종창、혼탁,공포화;신장간질비례증대,신소관화신소체수량명현감소,신소관상피세포종창、변성;비장내대량충혈,비장림파세포배사,H.E염색발현대량적종색“소결”충척우정개비장지중.
@@@@In the present study, the effects of Aeromonas hydrophila on the liver, spleen and kidney of the juve?nile grass carps were investigated by using histological methods. The results show that all the three organs (liv?er, spleen and kidney) of the infected fish were swelled at different degrees. The color of two organs changed, the liver was gray in color, and the spleen was dark purple in color. The internal structures of the liver were found badly destroyed, with fewer blood cells, swelling, turbid and vacuolization cells. The mesenchyme of kid?ney was enlarged, and the renal tubules and renal corpuscles were decreased obviously. The epithelial cells of re?nal tubules swelled and denatured. The necrosis of the spleen lymphocytes was found. Many brown granules were observed in spleen by H.E staining.