腐植酸
腐植痠
부식산
HUMIC ACID
2013年
2期
32-37
,共6页
Janka Va?ková%Beáta Velik%Martina Pilátová%Ivan Kron%Ladislav Va?ko%何静%丁亚芳%杨静%李宝才
Janka Va?ková%Beáta Velik%Martina Pilátová%Ivan Kron%Ladislav Va?ko%何靜%丁亞芳%楊靜%李寶纔
Janka Va?ková%Beáta Velik%Martina Pilátová%Ivan Kron%Ladislav Va?ko%하정%정아방%양정%리보재
腐植酸%线粒体%抗氧化酶%谷胱甘肽%细胞培养
腐植痠%線粒體%抗氧化酶%穀胱甘肽%細胞培養
부식산%선립체%항양화매%곡광감태%세포배양
humic acid%mitochondria%antioxidant enzyme%glutathione%cell culture
腐植酸被认为在动物喂饲试验中对健康和产率具有整体积极作用,同时颇有争议的是,其也被作为肿瘤的病原学因素之一.我们尝试评价斯洛伐克地区特定来源腐植酸在使用推荐预防剂量时的体外作用.研究其体外的抗氧化性质、对肝线粒体中酶系和非酶系抗氧化防御系统的作用以及对人工培养肿瘤细胞株的作用.我们观察到不论腐植酸是溶解于二甲基亚砜(DMSO)或是直接加入呼吸介质中线粒体悬浮液,在腐植酸给药后超氧化物歧化酶(SOD)活性显著下降.所测定的其他抗氧化酶如谷胱甘肽过氧化物酶(GPx)和谷胱甘肽还原酶(GR),其活性及还原型谷胱甘肽(GSH)的含量与对照组相比并未表现出显著变化.腐植酸对超氧阴离子自由基(O2-·)的抑制率低于其对羟自由基(·OH)的抑制率.6种不同肿瘤细胞株的存活率显示出只有急性T淋巴性白血病细胞株对测试腐植酸敏感.尽管在水溶液中溶解度相对较低,但特定来源腐植酸参与了氧化还原调控.通过夺取自由基,腐植酸重置了抗氧化防御机制.来源于自然资源的腐植酸体外研究结果显示出其作为有前景的免疫增强剂的潜力.
腐植痠被認為在動物餵飼試驗中對健康和產率具有整體積極作用,同時頗有爭議的是,其也被作為腫瘤的病原學因素之一.我們嘗試評價斯洛伐剋地區特定來源腐植痠在使用推薦預防劑量時的體外作用.研究其體外的抗氧化性質、對肝線粒體中酶繫和非酶繫抗氧化防禦繫統的作用以及對人工培養腫瘤細胞株的作用.我們觀察到不論腐植痠是溶解于二甲基亞砜(DMSO)或是直接加入呼吸介質中線粒體懸浮液,在腐植痠給藥後超氧化物歧化酶(SOD)活性顯著下降.所測定的其他抗氧化酶如穀胱甘肽過氧化物酶(GPx)和穀胱甘肽還原酶(GR),其活性及還原型穀胱甘肽(GSH)的含量與對照組相比併未錶現齣顯著變化.腐植痠對超氧陰離子自由基(O2-·)的抑製率低于其對羥自由基(·OH)的抑製率.6種不同腫瘤細胞株的存活率顯示齣隻有急性T淋巴性白血病細胞株對測試腐植痠敏感.儘管在水溶液中溶解度相對較低,但特定來源腐植痠參與瞭氧化還原調控.通過奪取自由基,腐植痠重置瞭抗氧化防禦機製.來源于自然資源的腐植痠體外研究結果顯示齣其作為有前景的免疫增彊劑的潛力.
부식산피인위재동물위사시험중대건강화산솔구유정체적겁작용,동시파유쟁의적시,기야피작위종류적병원학인소지일.아문상시평개사락벌극지구특정래원부식산재사용추천예방제량시적체외작용.연구기체외적항양화성질、대간선립체중매계화비매계항양화방어계통적작용이급대인공배양종류세포주적작용.아문관찰도불론부식산시용해우이갑기아풍(DMSO)혹시직접가입호흡개질중선립체현부액,재부식산급약후초양화물기화매(SOD)활성현저하강.소측정적기타항양화매여곡광감태과양화물매(GPx)화곡광감태환원매(GR),기활성급환원형곡광감태(GSH)적함량여대조조상비병미표현출현저변화.부식산대초양음리자자유기(O2-·)적억제솔저우기대간자유기(·OH)적억제솔.6충불동종류세포주적존활솔현시출지유급성T림파성백혈병세포주대측시부식산민감.진관재수용액중용해도상대교저,단특정래원부식산삼여료양화환원조공.통과탈취자유기,부식산중치료항양화방어궤제.래원우자연자원적부식산체외연구결과현시출기작위유전경적면역증강제적잠력.
Humic acids are known for their overall positive health and productivity effects in animal feeding trials and, controversially, as an aetiological factor of cancer. We tried to assess the in vitro effect of humic acids from a selected source in Slovakia when used at recommended prophylactic dosage. We investigated antioxidant properties, enzymatic and non-enzymatic antioxidant defence system in liver mitochondria and cultured cancer cell lines in vitro. We observed a significant decrease in superoxide dismutase activity after humic acids treatment irrespective of dissolving in dimethyl sulphoxide or direct addition to mitochondria suspension in a respiration medium. Activities of other antioxidant en-zymes measured, such as glutathione peroxidase and glutathione reductase, showed no significant differences from the control as well as the reduced glutathione content. Percentage of inhibition by humic acids of superoxide radical indicat-ed lower efficacy compared with that of hydroxyl radical. Survival of six different cancer cells lines indicated that only the acute T lymphoblastic leukaemia cell line was sensitive to the tested humic acids. Despite relatively low solubility in aqueous solutions, humic acids from the selected source participated in redox regulation. By recapturing the radicals, humic acids reloaded the antioxidant defensive mechanism. Results from in vitro study conducted with humic acids from the natural source showed potential of these substances as promising immunity enhancing agents.