食品工程
食品工程
식품공정
FOOD ENGINEERING
2013年
1期
53-56
,共4页
分散液液微萃取-高效液相色谱/质谱法%乳制品%氯霉素
分散液液微萃取-高效液相色譜/質譜法%乳製品%氯黴素
분산액액미췌취-고효액상색보/질보법%유제품%록매소
DLLME-LC/MS%dairy products%chloramphenicol
建立了分散液液微萃取-液相色谱/质谱法(DLLME-LC/MS)测定乳制品中氯霉素(CAP)残留的分析方法.采用单次单因子法对影响DLLME富集因子的实验参数进行了优化,试验结果表明,在最优DLLME条件下,方法检测限为0.2μg / kg,当CAP质量分数在1μg / kg~20μg / kg范围内线性关系良好(R2=0.9961),样品回收率(86%~94%)、方法精密度(2.7%~3.9%)均符合要求,该方法可用于乳制品中CAP残留的检测.
建立瞭分散液液微萃取-液相色譜/質譜法(DLLME-LC/MS)測定乳製品中氯黴素(CAP)殘留的分析方法.採用單次單因子法對影響DLLME富集因子的實驗參數進行瞭優化,試驗結果錶明,在最優DLLME條件下,方法檢測限為0.2μg / kg,噹CAP質量分數在1μg / kg~20μg / kg範圍內線性關繫良好(R2=0.9961),樣品迴收率(86%~94%)、方法精密度(2.7%~3.9%)均符閤要求,該方法可用于乳製品中CAP殘留的檢測.
건립료분산액액미췌취-액상색보/질보법(DLLME-LC/MS)측정유제품중록매소(CAP)잔류적분석방법.채용단차단인자법대영향DLLME부집인자적실험삼수진행료우화,시험결과표명,재최우DLLME조건하,방법검측한위0.2μg / kg,당CAP질량분수재1μg / kg~20μg / kg범위내선성관계량호(R2=0.9961),양품회수솔(86%~94%)、방법정밀도(2.7%~3.9%)균부합요구,해방법가용우유제품중CAP잔류적검측.
A method of determination of chloramphenicol in dairy products by Dispersive Liquid-Liquid Microextraction-Liquid Chromatography-Tandem Mass Spectrometry (DLLME-LC/MS)was developed. Enrichment factors that affect the extraction were optimized according to single factorial experiments. The Results showed that under the optimum DLLME conditions, the detection limits of the method was 0.2 μg/kg. The linearity of the method was R2=0.996 1 when the mass fraction of chloramphenicol was 1μg/kg~20μg/kg. The sample recoveries were between 86% and 94%. The relative standard deviations varied from 2.7% to 3.9%. This method can be applied to the determination of chloramphenicol in dairy products.
