CAJ | 학술논문

采用室内培养实验研究外加乳酸和蒽醌-2,6-磺酸钠等营养源条件下,玄武岩砖红壤中五氯酚的还原转化动力学与微生物群落结构变化.结果表明,玄武岩砖红壤中五氯酚的还原转化一级动力学常数为4.5×10?3 d?1;添加乳酸或同时添加乳酸和AQDS,五氯酚的还原转化速率显著提高,其一级动力学常数分别为7.3×10?3 d?1和14.3×10?3 d?1.循环伏安试测结果表明,添加乳酸和 AQDS 促进了砖红壤中活性亚铁的生成,并显著降低体系的氧化半反应峰电位.末端限制性片段长度多态性分析结果表明,添加五氯酚、乳酸和 AQDS 显著影响微生物群落结构,土壤微生物多样性显著下降;添加乳酸时,体系中微生物优势种群为梭菌,存在少量的具有铁还原能力的希瓦氏菌,未发现脱氯菌.因此,玄武岩砖红壤中五氯酚的还原转化主要是通过铁还原菌介导生成吸附态Fe(II)引起的活性亚铁还原脱氯机制.
채용실내배양실험연구외가유산화은곤-2,6-광산납등영양원조건하,현무암전홍양중오록분적환원전화동역학여미생물군락결구변화.결과표명,현무암전홍양중오록분적환원전화일급동역학상수위4.5×10?3 d?1;첨가유산혹동시첨가유산화AQDS,오록분적환원전화속솔현저제고,기일급동역학상수분별위7.3×10?3 d?1화14.3×10?3 d?1.순배복안시측결과표명,첨가유산화 AQDS 촉진료전홍양중활성아철적생성,병현저강저체계적양화반반응봉전위.말단한제성편단장도다태성분석결과표명,첨가오록분、유산화 AQDS 현저영향미생물군락결구,토양미생물다양성현저하강;첨가유산시,체계중미생물우세충군위사균,존재소량적구유철환원능력적희와씨균,미발현탈록균.인차,현무암전홍양중오록분적환원전화주요시통과철환원균개도생성흡부태Fe(II)인기적활성아철환원탈록궤제.
In this study, we investigated the reductive transformation of PCP and soil bacteria community structures in basalt latosol under addition of lactate and AQDS. Results showed that PCP reductive transformation occurred with k value of 4.5×10?3 d-1 in basalt latosol. The addition of lactate and AQDS led to the enhanced rates of PCP transformation with k value of 7.3×10?3 d?1 and 14.3 × 10?3 d?1, respectively. Cyclic voltammetry test results showed that the addition of lactate and AQDS led to the enhanced rates of active Fe(Ⅱ)species generation in basalt latosol and significantly enhance the reduction potential in the system. T-RFLP analysis results showed that the microbial communities were affected by the addition of PCP, lactate and AQDS and the microbial diversity was significantly decreased. Clostridum was the dominant population in the system after the addition of lactate. There were also small amounts of Shewanella which has iron-reducing ability but no dechlorinating bacteria. Therefore, reductive transformation of PCP in basalt latosol occurs though chemical dechlorinating process, which induced by iron-reducing bacteria indirectly.