CAJ | 학술논문

研究了大气压冷等离子体射流对子宫颈癌Hela细胞的灭活机制.在倒置显微镜下观察不同等离子体处理条件下的细胞形态,并通过中性红吸收测试定量测定各个条件下的细胞存活率.将功率维持在18 W,在900 mL/min氩等离子体中添入氧气的百分含量分别为1%,2%,4%和8%的条件下处理Hela细胞,探讨活性气体氧气在惰性气体氩气中的百分含量对Hela癌细胞灭活效率的影响,发现添加2%氧气时,氩/氧等离子体灭活效果最佳,处理180 s后细胞存活率可降至7%.当继续添加氧超过2%时,灭活效果逐渐减弱,直至8%时,其效果反而不如单纯氩等离子体.通过测量等离子体发射光谱,结果表明活性氧自由基在癌细胞灭活过程中可能起关键作用.
연구료대기압랭등리자체사류대자궁경암Hela세포적멸활궤제.재도치현미경하관찰불동등리자체처리조건하적세포형태,병통과중성홍흡수측시정량측정각개조건하적세포존활솔.장공솔유지재18 W,재900 mL/min아등리자체중첨입양기적백분함량분별위1%,2%,4%화8%적조건하처리Hela세포,탐토활성기체양기재타성기체아기중적백분함량대Hela암세포멸활효솔적영향,발현첨가2%양기시,아/양등리자체멸활효과최가,처리180 s후세포존활솔가강지7%.당계속첨가양초과2%시,멸활효과축점감약,직지8%시,기효과반이불여단순아등리자체.통과측량등리자체발사광보,결과표명활성양자유기재암세포멸활과정중가능기관건작용.
@@@@An inactivation mechanism study on Hela cancer cells by means of an atmospheric pressure cold plasma jet is presented. Cell morphology is observed under an inverted microscope after plasma treatment. The neutral red uptake assay provides quantitative evaluations of cell viability under different conditions. The effect of the inactivation efficiency of Hela cancer cells in the argon (900 mL/min) with addition of different amount of oxygen (1%, 2%, 4%, 8%) into atmospheric pressure cold plasma jet is discussed under the fixed power 18 W. Results show that 2% O2 addition provides the best inactivation efficiency, and the survival rate can be reduced to 7%after 180 s treatment. When the oxygen addition exceeds 2%, the inactivation efficiency gradually weakens. The effect is not so good as that in pure argon plasma when the oxygen addition arrives at 8%. According to the emission spectrum of the plasmum, it is concluded that the reactive oxygen species in the plasma play a key role in cancer cell inactivation process.