CAJ | 학술논문

应用酶消化和机械吹打相结合的方法,快速分离出中华乌塘鳢(Bostrychys sinensis)嗅囊中纤毛嗅觉感受神经元.再应用全细胞膜片钳技术记录中华乌塘鳢纤毛嗅觉感受神经元的静息电位、动作电位及电压门控离子通道电流等电生理学特征.结果表明,中华乌塘鳢纤毛嗅觉感受神经元具有典型的双级神经元结构特征,包括细胞体,轴突,树突以及树突顶端的嗅结和纤毛.嗅觉感受神经元静息电位为(?57±6.37) mV,动作电位峰值为(38.30±0.95) mV.在?60~+80 mV 的阶梯去极化脉冲刺激中,内向电流在?60~?40 mV 激活,?20~0 mV 达到最大值,内向电流峰值为(?69.08±44.15) pA.外向电流在+40 mV 时达最大值,峰值为(267.55±73.67) pA.本研究结果为纤毛嗅觉感受神经元上性信息素受体感受性信息素的电生理机制以及性信息素的应用提供了方法和理论基础.
응용매소화화궤계취타상결합적방법,쾌속분리출중화오당례(Bostrychys sinensis)후낭중섬모후각감수신경원.재응용전세포막편겸기술기록중화오당례섬모후각감수신경원적정식전위、동작전위급전압문공리자통도전류등전생이학특정.결과표명,중화오당례섬모후각감수신경원구유전형적쌍급신경원결구특정,포괄세포체,축돌,수돌이급수돌정단적후결화섬모.후각감수신경원정식전위위(?57±6.37) mV,동작전위봉치위(38.30±0.95) mV.재?60~+80 mV 적계제거겁화맥충자격중,내향전류재?60~?40 mV 격활,?20~0 mV 체도최대치,내향전류봉치위(?69.08±44.15) pA.외향전류재+40 mV 시체최대치,봉치위(267.55±73.67) pA.본연구결과위섬모후각감수신경원상성신식소수체감수성신식소적전생리궤제이급성신식소적응용제공료방법화이론기출.
@@@@The ciliated olfactory receptor neurons (cORNs) in the olfactory sac were dissociated from Bostrichthys sinensis by enzymatic and mechanical methods. The whole-cell patch-clamp technique was used to determine the resting potential, action potential and voltage-gated ion channel current. The results showed that the B. sinensis cORN was a bipolar neuron, containing cell body, axon and dendrites with olfactory knobs and cilia. The resting and action potential peaks of ciliated olfactory receptor neurons were (–57 ± 6.37) mV and (38.30 ± 0.95) mV, respec-tively. In response to depolarizing voltage steps pulse stimulation from –60 ~ +80 mV, the transient inward current was activated between –60 mV and –40 mV, and reached a peak averaging (–69.08 ± 44.15) pA at ?20 to 0 mV; the outward current reached a peak averaging (267.55 ± 73.67) pA at +40 mV. The present study provides a base theory for research in the electrophysiological mechanisms of sex pheromones with their receptors and the application of sex pheromones.