中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
12期
9-11
,共3页
姜黄素%宫颈癌%p53mRNA%p53蛋白
薑黃素%宮頸癌%p53mRNA%p53蛋白
강황소%궁경암%p53mRNA%p53단백
Curcumin%Cervical cancer%p53mRNA%p53 protein
目的:主要探讨姜黄素对人类宫颈癌Hela细胞中增殖作用的影响,以及细胞p53蛋白表达的影响.方法:本文通过不同浓度姜黄素干预人宫颈癌Hela细胞,采用MTT法检测药物对Hela细胞的增殖抑制率;RT-PCR和Western blot检测细胞凋亡相关蛋白p53表达.结果:MTT发现姜黄素对人宫颈癌Hela细胞有一定的抑制作用,并呈时间-剂量依赖性;RT-PCR结果显示p53mRNA的表达增加;western boltting显示p53蛋白的表达增加.结论:姜黄素可一直Hela细胞增殖,其机制可能是通过增加p53基因的表达而实现.
目的:主要探討薑黃素對人類宮頸癌Hela細胞中增殖作用的影響,以及細胞p53蛋白錶達的影響.方法:本文通過不同濃度薑黃素榦預人宮頸癌Hela細胞,採用MTT法檢測藥物對Hela細胞的增殖抑製率;RT-PCR和Western blot檢測細胞凋亡相關蛋白p53錶達.結果:MTT髮現薑黃素對人宮頸癌Hela細胞有一定的抑製作用,併呈時間-劑量依賴性;RT-PCR結果顯示p53mRNA的錶達增加;western boltting顯示p53蛋白的錶達增加.結論:薑黃素可一直Hela細胞增殖,其機製可能是通過增加p53基因的錶達而實現.
목적:주요탐토강황소대인류궁경암Hela세포중증식작용적영향,이급세포p53단백표체적영향.방법:본문통과불동농도강황소간예인궁경암Hela세포,채용MTT법검측약물대Hela세포적증식억제솔;RT-PCR화Western blot검측세포조망상관단백p53표체.결과:MTT발현강황소대인궁경암Hela세포유일정적억제작용,병정시간-제량의뢰성;RT-PCR결과현시p53mRNA적표체증가;western boltting현시p53단백적표체증가.결론:강황소가일직Hela세포증식,기궤제가능시통과증가p53기인적표체이실현.
Objective:To observe the effect of cuicumin on proliferation of human cervical cancer Hela cells and expression of p53 protein. Method:In this paper,Hela cells were treated with curcumin at various concentrations for various hours,Then determined for proliferation activity by MTT method,for transcription levels ofβ-catenin and p53 mRNAs by RT-PCR,and for expressions ofβ-catenin and p53 protein by Western blot. Result:Curcumin showed dose-and time-dependent inhibitory effect on the proliferation of Hela cells.Meanwhile,curcumin increased the mRNA transcription and protein expression levels ofβ-catenin and p53.Conclusion:Curcumin inhibited the proliferation of Hela cells by a possible mechanism of increased the express of p53.