CAJ | 학술논문

目的通过胆固醇结石(GS)小鼠肝脏的核受体基因—肝核受体类似物1(1iver receptor homolog 1,LRH-1)、法尼醇受体(farnesoid X receptor,FXR)、类固醇受体(steroid X receptor,SXR)的表达,探讨与胆固醇结石形成关系.方法 C57BL/6雌性小鼠40只,平均出生后10周,重量28~35g,20只一组分为两组,一组予以正常饮食另一组予以高脂饮食喂养,均8周后处死.病理切片检查胆囊壁细胞组织学改变,并分别采用实时定量RT-PCR法和Western Blot法测定肝脏LRH-I、FXR、SXR的mRNA表达.结果胆固醇结石小鼠胆囊壁细胞呈现炎性改变,LRH-I的mRNA表达均高于对照组(P<0.01),FXR、SXR的mRNA表达两组差异无统计学意义.结论啮齿动物肝脏LRH-1基因的表达异常与胆固醇结石形成有关,FXR、SXR基因的表达与胆固醇结石形成可能无关.
목적통과담고순결석(GS)소서간장적핵수체기인—간핵수체유사물1(1iver receptor homolog 1,LRH-1)、법니순수체(farnesoid X receptor,FXR)、류고순수체(steroid X receptor,SXR)적표체,탐토여담고순결석형성관계.방법 C57BL/6자성소서40지,평균출생후10주,중량28~35g,20지일조분위량조,일조여이정상음식령일조여이고지음식위양,균8주후처사.병리절편검사담낭벽세포조직학개변,병분별채용실시정량RT-PCR법화Western Blot법측정간장LRH-I、FXR、SXR적mRNA표체.결과담고순결석소서담낭벽세포정현염성개변,LRH-I적mRNA표체균고우대조조(P<0.01),FXR、SXR적mRNA표체량조차이무통계학의의.결론교치동물간장LRH-1기인적표체이상여담고순결석형성유관,FXR、SXR기인적표체여담고순결석형성가능무관.
Objective To investigate the expressions of nuclear receptor gene-1iver receptor homolog 1(LRH-1),farnesoid X receptor(FXR)and steroid xenobiotic receptor(SXR)gene in cholesterol gallstone(CGS)mice. Methods Forty C57BL/6 mice,20 with cholesterol gallstone(GS)and 20 controls without gallstones(GSF)were enrolled in this study. mRNA and protein expression of LRH-1、FXR and SXR genes were determined by RT-PCR and Western Blot. Results Gallbladder walls of GS mice were thicker with increased stromal granulocyte infiltration. The expression levels of LRH-1 genes were significantly higher in GS mice than in controls(LRH-1:P<0.01).But There were no significant differences in protein and mRNA expressions of FXR and SXR gene between two groups. Conclusion The increased expression of LRH-1 gene may be related to GS disease.