浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2013年
7期
529-532
,共4页
张浩%郭治宇%胡桂英%周绍斌%许多嘉%施红旗%樊静%何忠平%金志刚%吴波%洪毅%程俊峰%陆磊
張浩%郭治宇%鬍桂英%週紹斌%許多嘉%施紅旂%樊靜%何忠平%金誌剛%吳波%洪毅%程俊峰%陸磊
장호%곽치우%호계영%주소빈%허다가%시홍기%번정%하충평%금지강%오파%홍의%정준봉%륙뢰
深静脉血栓形成%肺栓塞%CT血管造影%B超%小型猪
深靜脈血栓形成%肺栓塞%CT血管造影%B超%小型豬
심정맥혈전형성%폐전새%CT혈관조영%B초%소형저
Deep venous thrombosis%Pulmonary embolism%Computed tomographic angiography%B-mode ultra-sound%Piglet
目的初步探寻建立下肢深静脉血栓形成(DVT)合并肺栓塞(PE)的小型猪模型的方法.方法15只小型猪随机分为3组(A、B、C).每组通过结扎双侧股静脉并注入氨甲苯酸以获得DVT模型,并经B超证实;3组实验猪分别于建模后第1、4、7天拆除双侧结扎线,并洗脱一侧股静脉内自体血栓后行CT血管造影(CTA)检查,若无肺栓塞影像学证据,则经头静脉注入体外制备的血栓后再次行CTA检查,7d后将小型猪处死并行病理学检查.结果2只小型猪因严重肺炎排除,其余13只中11只DVT合并PE建模成功并经病理证实,建模即刻成功率73.33%(7d后成功率53.33%),其中B组建模成功率为100%.其中11只DVT在B超上表现为股静脉腔内的条索状低回声;PE在CTA上表现为肺动脉主干或分支的充盈缺损及截断.病理检查示肺动脉内见混有明胶海绵和自体血栓的栓塞物.结论下肢DVT合并PE小型猪模型可成功建立;B超和CTA检查可以较好的评价DVT和PE.
目的初步探尋建立下肢深靜脈血栓形成(DVT)閤併肺栓塞(PE)的小型豬模型的方法.方法15隻小型豬隨機分為3組(A、B、C).每組通過結扎雙側股靜脈併註入氨甲苯痠以穫得DVT模型,併經B超證實;3組實驗豬分彆于建模後第1、4、7天拆除雙側結扎線,併洗脫一側股靜脈內自體血栓後行CT血管造影(CTA)檢查,若無肺栓塞影像學證據,則經頭靜脈註入體外製備的血栓後再次行CTA檢查,7d後將小型豬處死併行病理學檢查.結果2隻小型豬因嚴重肺炎排除,其餘13隻中11隻DVT閤併PE建模成功併經病理證實,建模即刻成功率73.33%(7d後成功率53.33%),其中B組建模成功率為100%.其中11隻DVT在B超上錶現為股靜脈腔內的條索狀低迴聲;PE在CTA上錶現為肺動脈主榦或分支的充盈缺損及截斷.病理檢查示肺動脈內見混有明膠海綿和自體血栓的栓塞物.結論下肢DVT閤併PE小型豬模型可成功建立;B超和CTA檢查可以較好的評價DVT和PE.
목적초보탐심건립하지심정맥혈전형성(DVT)합병폐전새(PE)적소형저모형적방법.방법15지소형저수궤분위3조(A、B、C).매조통과결찰쌍측고정맥병주입안갑분산이획득DVT모형,병경B초증실;3조실험저분별우건모후제1、4、7천탁제쌍측결찰선,병세탈일측고정맥내자체혈전후행CT혈관조영(CTA)검사,약무폐전새영상학증거,칙경두정맥주입체외제비적혈전후재차행CTA검사,7d후장소형저처사병행병이학검사.결과2지소형저인엄중폐염배제,기여13지중11지DVT합병PE건모성공병경병리증실,건모즉각성공솔73.33%(7d후성공솔53.33%),기중B조건모성공솔위100%.기중11지DVT재B초상표현위고정맥강내적조색상저회성;PE재CTA상표현위폐동맥주간혹분지적충영결손급절단.병리검사시폐동맥내견혼유명효해면화자체혈전적전새물.결론하지DVT합병PE소형저모형가성공건립;B초화CTA검사가이교호적평개DVT화PE.
@@@@Objective To establish a piglet model of deep venous thrombosis (DVT) and pulmonary embolism (PE). Methods The deep venous thrombosis was induced in 15 piglets by ligation of bilateral femoral vein and injection of aminomethylbenzoic acid, the DVT was confirmed by ultrasonography. DVT model piglets were randomly divided into 3 groups (n=5 in each), the ligation of femoral vein was released after 1d (group A), 4d (group B) and 7d (group C), respectively. Then au-tologous blood clots of one side of the femoral vein were flushed and the lungs were examined with CT angiography (CTA) scan. If there was no evidence of pulmonary embolism, in vitro prepared autologous blood clots and gelatin sponge were injected through the cephalic vein to induce the PE;and the established DVT and PE were confirmed by CTA. The piglets were sacrificed after 7d, and the pathological examinations were performed. Results The DVT and PE were successful y induced in 11 piglets with the success rate of 73.3%(53.3%after 7d), while the success rate in group B was 100.0%. The DVT was presented on ultra-sonography as endovenous strip-like hypoechoic foci. The presentation of PE on the CTA was fil ing-defect and truncation of the pulmonary trunk or branch. Pathological examination revealed pulmonary emboli mixed with gelatin sponge and autologous blood clots. Conclusion The piglet model of DVT and PE has been successful y established, which can be confirmed by ultrasonogra-phy and CTA.
