CAJ | 학술논문

目的探讨雌激素受体(ER)和孕激素受体(PR)在子宫腺肌病模型病灶中的表达及临床意义.方法用异体垂体移植法建立ICR小鼠子宫腺肌病模型,应用免疫组化SP法检测正常子宫内膜、模型子宫内膜及病灶异位子宫内膜中ER、PR的表达.结果垂体移植4个月和6个月后,均成功诱发子宫腺肌病模型,不同时间诱发模型的病灶大体结节数和HE染色评分之间比较,均无统计学差异(均P>0.05);建模6个月后模型病灶的结节显著大于建模4个月后(P<0.05).建模后4个月,模型子宫内膜和病灶异位子宫内膜以及正常子宫内膜中ER和PR表达的阳性率比较,均无统计学差异(均P>0.05);建模后6个月,3种子宫内膜中ER阳性率分别为55.07%、31.63%和24.74%;PR阳性率为48.40%、27.48%和22.70%;3者比较均有统计学差异(均P<0.05).结论子宫腺肌病的发生、发展可能与子宫内膜局部ER、PR的高表达相关.
목적탐토자격소수체(ER)화잉격소수체(PR)재자궁선기병모형병조중적표체급림상의의.방법용이체수체이식법건립ICR소서자궁선기병모형,응용면역조화SP법검측정상자궁내막、모형자궁내막급병조이위자궁내막중ER、PR적표체.결과수체이식4개월화6개월후,균성공유발자궁선기병모형,불동시간유발모형적병조대체결절수화HE염색평분지간비교,균무통계학차이(균P>0.05);건모6개월후모형병조적결절현저대우건모4개월후(P<0.05).건모후4개월,모형자궁내막화병조이위자궁내막이급정상자궁내막중ER화PR표체적양성솔비교,균무통계학차이(균P>0.05);건모후6개월,3충자궁내막중ER양성솔분별위55.07%、31.63%화24.74%;PR양성솔위48.40%、27.48%화22.70%;3자비교균유통계학차이(균P<0.05).결론자궁선기병적발생、발전가능여자궁내막국부ER、PR적고표체상관.
@@@@Objective To investigate the expression estrogen receptor (ER) and progesterone receptor (PR) in rice ade-nomyosis model and its significance. Methods The adenomyosis model was induced by al ogeneic pituitary transplantation in I-CR mice. The expression of ER and PR in endometrial tissue was detected by immunohistochemistry SP method. Results Ade-nomyosis was induced successful y by allogeneic pituitary transplantation in ICR mice 4 months and 6 months after transplanta-tion. There were no differences in the number of nodules and the grades of H&E staining between 4 and 6 months after transplan-tation (P>0.05). But the size of nodules at 6 months was larger than that at 4 months (P<0.05). At 6 months after transplantation, the positive rate of ER and PR in endometrial tissue or ectopic endometrial tissue were higher than that in normal endometrial tis-sue (P<0.05);however, the difference was not observed at 4 months after transplantation. Conclusion The expression of ER and PR in endometrial tissue is associated with the development of adenomyosis in ICR mice model.