CAJ | 학술논문

S100A4是S100蛋白家族的成员,在细胞的增殖、分化、损伤修复以及肿瘤细胞转移等方面发挥重要的调控作用.本研究将S100A4全长基因构建到pET28a原核表达载体上,利用大肠杆菌表达系统表达和纯化出高纯度的重组人S100A4.通过试验证明,重组人S100A4蛋白在体外可以有效地增强黑色素瘤细胞A375-S2的增殖.重组人S100A4原核表达与纯化方法的建立将促进其结构和生物学功能研究,并且对于S100蛋白家族其它蛋白的表达与纯化具有重要的参考意义.
S100A4시S100단백가족적성원,재세포적증식、분화、손상수복이급종류세포전이등방면발휘중요적조공작용.본연구장S100A4전장기인구건도pET28a원핵표체재체상,이용대장간균표체계통표체화순화출고순도적중조인S100A4.통과시험증명,중조인S100A4단백재체외가이유효지증강흑색소류세포A375-S2적증식.중조인S100A4원핵표체여순화방법적건립장촉진기결구화생물학공능연구,병차대우S100단백가족기타단백적표체여순화구유중요적삼고의의.
S100A4 is a member of S100 protein family, which exerts functions in cell proliferation, differention and cell damage repair. It is also reported that S100A4 is assoicated with cancer metastasis. To facilitate further studies of S100A4, we developed a strategy for expressing and purifying recombinant human S100A4 protein using E. coli expression system and affinity chromotography methods. This strategy is simple and efficient for purifying recombinant S100A4 with high purity and low endotoxin contamination. Furthermore, in vitro studies validated the biological activity of the purified S100A4 since it could enhance the viability of A375 cells. We hope that this strategy could facilitate the physiological and pathological research of S100A4 and have reference values for the expression and purification of other S100 family members.