CAJ | 학술논문

基因转录调节是植物对非生物胁迫适应机制的一个重要方面,转录调节因子在胁迫信号转导途径中调节下游基因的表达,在建立植物对胁迫适应性过程中起到重要作用.锌指蛋白是功能多样的转录调节因子蛋白家族,家族成员在植物响应非生物胁迫方面扮演着重要角色.本研究以秋茄C2H2型锌指蛋白编码基因KcZFP为目的基因,在烟草中过表达KcZFP,分析C2H2型锌指蛋白在植物耐盐性中的作用.研究结果显示:转基因株系中,KcZFP表达量显著提高.过表达KcZFP的烟草植株的耐盐性明显提高,在200 mmol/L NaCl处理的条件下,KcZFP过表达烟草中脯氨酸水平远高于野生型植株.对光合作用参数比较分析显示,在KcZFP过表达植株中净光合速率受盐胁迫的影响小于野生型植株,光合系统在一定程度上得到了保护.研究结果说明KcZFP作为转录调节因子参与了植物的渗透调节,对植物的耐盐性具有贡献.
기인전록조절시식물대비생물협박괄응궤제적일개중요방면,전록조절인자재협박신호전도도경중조절하유기인적표체,재건립식물대협박괄응성과정중기도중요작용.자지단백시공능다양적전록조절인자단백가족,가족성원재식물향응비생물협박방면분연착중요각색.본연구이추가C2H2형자지단백편마기인KcZFP위목적기인,재연초중과표체KcZFP,분석C2H2형자지단백재식물내염성중적작용.연구결과현시:전기인주계중,KcZFP표체량현저제고.과표체KcZFP적연초식주적내염성명현제고,재200 mmol/L NaCl처리적조건하,KcZFP과표체연초중포안산수평원고우야생형식주.대광합작용삼수비교분석현시,재KcZFP과표체식주중정광합속솔수염협박적영향소우야생형식주,광합계통재일정정도상득도료보호.연구결과설명KcZFP작위전록조절인자삼여료식물적삼투조절,대식물적내염성구유공헌.
Transcriptional regulation is one of the important factors in plant adaption to abiotic stress. In stress signaling pathway, transcription factors regulate downstream genes and play a critical role in the plant acclimation to adverse environments. Among transcription factors involved in abiotic stress response, zinc finger proteins represent a large protein family which displays diverse roles in various biological processes. In this study, the function of a KcZFP gene, encoding a Cys2/His2-type zinc-finger protein of Kandelia candel, was investigated in tobacco plants under salt treatment. The results showed that the transcript abundance of KcZFP was markedly elevated in transgenic plants. Salinity tolerance of KcZFP-transgenic plants was tested under 200 mmol/L NaCl. KcZFP-overexpressed tobacco plants exhibited an obviously higher salt tolerance than that of the wild type. The proline concentration in leaves of transgenic plants was markedly higher than that in wild-type under 200 mmol/L NaCl treatment. Moreover, net photosynthetic rate in KcZFP-overexpressed tobacco was less affected by salinity stress, as compared to the wild type. This result suggested that the elevated proline should exert a protective effect on the photosynthetic system. Therefore, our results showed that KcZFP functioned as a transcript regulator contributing to osmotic regulation in tobacco plants under NaCl stress.