CAJ | 학술논문

本试验旨在研究贵州白山羊GFI1B基因第八外显子的多态性,及其与生长性状的相关性.通过PCR-RFLP技术对贵州白山羊外显子SNPs位点进行检测,利用一般线形模型分析其与生长性状的关联性.结果显示,供试群体中在外显子上检测到2个SNPs位点,即第八外显子263(G/T)和340(G/A).最小二乘法分析表明,G340A位点,CC型和DD型的体重、体长、胸深和胸宽对CD型达到差异及显著水平(p约0.01).本研究检测到的GFI1B基因第八外显子340(G/A)多态性位点可作为贵州白山羊生长性状的候选分子标记.
본시험지재연구귀주백산양GFI1B기인제팔외현자적다태성,급기여생장성상적상관성.통과PCR-RFLP기술대귀주백산양외현자SNPs위점진행검측,이용일반선형모형분석기여생장성상적관련성.결과현시,공시군체중재외현자상검측도2개SNPs위점,즉제팔외현자263(G/T)화340(G/A).최소이승법분석표명,G340A위점,CC형화DD형적체중、체장、흉심화흉관대CD형체도차이급현저수평(p약0.01).본연구검측도적GFI1B기인제팔외현자340(G/A)다태성위점가작위귀주백산양생장성상적후선분자표기.
This study aimed at studying on the polymorphism of the eighth exons of GFI1B gene in Guizhou white goats and conducted its association analysis with growth traits. PCR-RFLP was applied to detect the polymorphism loci and the general linear model was used to analyze its association with growth traits. We found out two consistent SNPs sites in the eighth exon of GFI1B gene in the tested breeds, which are 263 bp G/T and 340 bp G/A. The following fixed effects model was employed in analyzing the growth trait in goat. 340 (G/C) sites was obviously significant that Body weight、Body length、Chest depth and Chest breadth difference between genotype CD and CC and DD (p<0.01). The 340 (G/C) sites might provide a study basis between GFI1B gene and growth traits as well as certain theoretical foundation of better feeding and genetic resources of indigenous goats.