CAJ | 학술논문

本研究采用免疫荧光及免疫组化DAB显色技术来检测Myogenin蛋白在五指山猪和长白猪胎儿和成年猪的骨骼肌中的定位和表达情况,并通过平均光密度统计分析Myogenin蛋白在五指山猪和长白猪胎儿和成年猪骨骼肌中表达水平.结果表明:Myogenin蛋白表达定位于骨骼肌细胞核表达;在五指山猪和长白猪胎儿和成年猪的腿部骨骼肌和背部骨骼肌中均检测到阳性产物,在猪胎儿期间骨骼肌中Myogenin蛋白的表达水平高于其在成熟猪骨骼肌中的表达;且在同种猪腿部骨骼肌中的表达水平显著高于其在背部骨骼肌中的表达;此外还检测到Myogenin蛋白在五指山猪骨骼肌中表达均显著高于其在长白猪骨骼肌中的表达(p<0.05).本研究得到的Myogenin蛋白在五指山猪和长白猪骨骼肌中存在显著的表达差异结果,为今后研究不同猪种间骨骼肌发育差异及探讨五指山猪矮小机制和发育机理提供了理论基础.
본연구채용면역형광급면역조화DAB현색기술래검측Myogenin단백재오지산저화장백저태인화성년저적골격기중적정위화표체정황,병통과평균광밀도통계분석Myogenin단백재오지산저화장백저태인화성년저골격기중표체수평.결과표명:Myogenin단백표체정위우골격기세포핵표체;재오지산저화장백저태인화성년저적퇴부골격기화배부골격기중균검측도양성산물,재저태인기간골격기중Myogenin단백적표체수평고우기재성숙저골격기중적표체;차재동충저퇴부골격기중적표체수평현저고우기재배부골격기중적표체;차외환검측도Myogenin단백재오지산저골격기중표체균현저고우기재장백저골격기중적표체(p<0.05).본연구득도적Myogenin단백재오지산저화장백저골격기중존재현저적표체차이결과,위금후연구불동저충간골격기발육차이급탐토오지산저왜소궤제화발육궤리제공료이론기출.
The immunofluorescence and immunohistochemistry DAB color method were used to detect the expression of Myogenin protein of Wuzhishan pig and Landrace pig's skeletal muscle in fetal period and mature stage, and the IOD of the statistical analysis used to analyze the Myogenin protein expression level of Wuzhishan pig and Landrace pig's skeletal muscle in fetal period and mature stage. The results showed that Myogenin protein was expressed in nucleus of skeletal muscle. Myogenin protein expressed in skeletal muscle in fetal period and mature stage of Wuzhishan pig and Landrace pig. The higher expression level of Myogenin protein in skeletal muscle in fetal period than it in mature stage, the higher expression level of Myogenin protein in leg skeletal muscle than it in back skeletal muscle, and in addition detected that the higher expression level of Myogenin protein in Wuzhishan pig than it in landrace pig (p<0.05). Myogenin protein expression was significantly different in skeletal muscle of Wuzhishan pig and landrace pig. The results provided a theoretical basis for future study on the dwarf mechanism and development of Wuzhishan pig.