CAJ | 학술논문

从5龄家蚕肠道分离筛选得到一株产纤维素酶菌株BMC-2,以羧甲基纤维素酶(CMCase)比活力为响应值,通过Plackett-Burman试验设计、最陡爬坡试验和Box-Behnken试验设计对菌株BMC-2产纤维素酶发酵条件进行优化,结果表明,发酵时间、发酵温度、培养基初始pH和转速对CMCase比活力具有显著影响,其影响程度由大到小依次为发酵时间、培养基初始pH、发酵温度、转速.确定菌株BMC-2产纤维素酶最优发酵条件为:发酵时间94.35 h,发酵温度30.3℃,培养基初始pH 7.01,转速179 r/min.在此条件下, CMCase比活力理论值为25.801 U/mg,验证值为25.526 U/mg,较产酶条件优化前提高了1倍,预测模型可靠性高,可应用于菌株BMC-2产纤维素酶条件的优化.
종5령가잠장도분리사선득도일주산섬유소매균주BMC-2,이최갑기섬유소매(CMCase)비활력위향응치,통과Plackett-Burman시험설계、최두파파시험화Box-Behnken시험설계대균주BMC-2산섬유소매발효조건진행우화,결과표명,발효시간、발효온도、배양기초시pH화전속대CMCase비활력구유현저영향,기영향정도유대도소의차위발효시간、배양기초시pH、발효온도、전속.학정균주BMC-2산섬유소매최우발효조건위:발효시간94.35 h,발효온도30.3℃,배양기초시pH 7.01,전속179 r/min.재차조건하, CMCase비활력이론치위25.801 U/mg,험증치위25.526 U/mg,교산매조건우화전제고료1배,예측모형가고성고,가응용우균주BMC-2산섬유소매조건적우화.
A novel cellulase-producing fungus named BMC-2 was isolated from the fifth-instar larval gut of Bom-byx mori. Fermentation conditions for the cellulase-producing, in which CMCase specific activity was taken as re-sponse value, were optimized with Plackett-Burman design, steepest ascent test and Box-Benhken design. The re-sults showed that fermentation time, fermentation temperature, initial pH of medium and rotational speed had influ-ence on cellulase-producing significantly. The affecting order was fermentation time, initial pH of medium, fermen-tation temperature and rotational speed, in turn. The optimized fermentation conditions were as follows:fermenta-tion time 94.35 h, fermentation temperature 30.3℃, initial pH of medium 7.01 and rotational speed 179 r/min. Under the optimal conditions, the CMCase specific activity was 25.801 U/mg in theory and 25.526 U/mg in actual value that was increased by 1 times compared with the initial fermentation condition. The result indicated that the established mathematical model by response surface methodology was feasible for practical prediction.