CAJ | 학술논문

以PCR产物克隆后测序的方法测定了贵州赤水桫椤国家级自然保护区3个种群59个个体的叶绿体DNA (cpDNA) atpB-rbcL非编码区序列.序列长度介于727~732 bp之间,具长度多态性,A+T百分比含量较高,介于63.27%~63.89%之间.遗传多样性水平较低,表现出较高的单倍型多样性(h=0.639)和低的核苷酸多样性(Dij=0.00028)并存的特征,提示现有种群可能源自一个有效群体规模较小的种群的快速扩张,扩张时间尚短,不足以形成更为复杂的遗传结构.种群间的高基因流Nm、低遗传分化度FST、AMOVA分析以及DNA歧异度结果一致显示各种群间无明显的遗传分化.
이PCR산물극륭후측서적방법측정료귀주적수사라국가급자연보호구3개충군59개개체적협록체DNA (cpDNA) atpB-rbcL비편마구서렬.서렬장도개우727~732 bp지간,구장도다태성,A+T백분비함량교고,개우63.27%~63.89%지간.유전다양성수평교저,표현출교고적단배형다양성(h=0.639)화저적핵감산다양성(Dij=0.00028)병존적특정,제시현유충군가능원자일개유효군체규모교소적충군적쾌속확장,확장시간상단,불족이형성경위복잡적유전결구.충군간적고기인류Nm、저유전분화도FST、AMOVA분석이급DNA기이도결과일치현시각충군간무명현적유전분화.
Chloroplast DNA (cpDNA) atpB-rbcL noncoding sequence of Alsophila spodophylla, collected from three populations distributed at Asphole National Nature Reserve in Chishui of Guizhou province were sequenced. Sequence length varied from 726~732 bp, showing length polymorphism, with high A+T content between 63.27%and 63.89%. High level of haplotype diversity (h=0.639) and a low nucleotide diversity (Dij=0.000 28) were detected in the populations of Alsophila spinulosa. This suggested rapid demographic expansion from a small effective population, and, since then, there has been insufficient time to form a more complicated population structure. Observed high gene flow Nm, low FST, AMOVA analysis and DNA divergence data consistently indicated that no genetic differentiation should occur at the population level.