CAJ | 학술논문

为了利用分子标记技术研究湖北海棠的起源、系统发育和分类问题,本研究对湖北海棠及其近缘种叶DNA的CTAB提取法进行改良,并对SSR体系优化.结果表明,将EDTA用量从20 mmol/L提高到80 mmol/L,加大PVP和RNA酶的用量,有助于提高从湖北海棠叶中提取的DNA质量;利用反复实验优化的SSR体系,可从40对引物中筛选出18对多态性好、条带清晰的湖北海棠SSR引物.本研究筛选出的多态引物,将为探讨湖北海棠的起源、进化和遗传变异等提供基础资料.
위료이용분자표기기술연구호북해당적기원、계통발육화분류문제,본연구대호북해당급기근연충협DNA적CTAB제취법진행개량,병대SSR체계우화.결과표명,장EDTA용량종20 mmol/L제고도80 mmol/L,가대PVP화RNA매적용량,유조우제고종호북해당협중제취적DNA질량;이용반복실험우화적SSR체계,가종40대인물중사선출18대다태성호、조대청석적호북해당SSR인물.본연구사선출적다태인물,장위탐토호북해당적기원、진화화유전변이등제공기출자료.
In order to study the origin, systematic development and the taxonomic problems of M. hupehensis, the CTAB extraction methods for DNA of M. hupehensis and several related species were improved in this paper and the SSR system has been optimized. The results showed that it is helpful to improve the DNA quality which was extracted from leaves of M. hupehensis by increasing the concentration of EDTA from 20 mmol/L to 80 mmol/L, the amount of PVP and RNA enzyme. Through the optimal SSR system, 18 primers with high polymorphism were selected from 40 primers. These polymorphic primers will provide the basic information for the origin, evolution, and genetic variation of M. hupehensis.