CAJ | 학술논문

为了分析鲨烯合酶(squalene synthase, SS)基因密码子的使用方式及其影响因素,利用codon W和SPSS 16.0软件对47条来自不同物种的SS基因进行多元统计分析、对应性分析.SS基因密码子1~3位碱基的GC含量(GC1, GC2和GC3)依次为51.33%、34.65%和54.37%,3个位点的GC含量均呈极显著相关关系(p<0.01),对应性分析的结果表明,第1轴显示30.71%的差异,有效密码子数和GC3、GC1和GC2的均值与GC3之间的相关性均达极显著水平(p<0.01).筛选出的26个最优密码子的第3位碱基均为G或C.以MEGA 5.0构建的基于SS蛋白质序列的进化树比基于RSCU的聚类更符合传统的系统发育观点.SS基因密码子偏好以G/C结尾,使用模式受选择和突变影响,突变对密码子偏好影响较大.
위료분석사희합매(squalene synthase, SS)기인밀마자적사용방식급기영향인소,이용codon W화SPSS 16.0연건대47조래자불동물충적SS기인진행다원통계분석、대응성분석.SS기인밀마자1~3위감기적GC함량(GC1, GC2화GC3)의차위51.33%、34.65%화54.37%,3개위점적GC함량균정겁현저상관관계(p<0.01),대응성분석적결과표명,제1축현시30.71%적차이,유효밀마자수화GC3、GC1화GC2적균치여GC3지간적상관성균체겁현저수평(p<0.01).사선출적26개최우밀마자적제3위감기균위G혹C.이MEGA 5.0구건적기우SS단백질서렬적진화수비기우RSCU적취류경부합전통적계통발육관점.SS기인밀마자편호이G/C결미,사용모식수선택화돌변영향,돌변대밀마자편호영향교대.
To analyze the codon usage of squalene synthase (SS) gene and the influence factors, multivariate ana-lysis and corresponding analysis of SS genes from different species were analyzed by software codon W and SPSS 16.0. GC content at three positions of codons (GC1, GC2 and GC3) in turns was 51.33%、34.65%and 54.37%, and corresponded significantly. GC1 and GC2 have a significant correlation coefficient (p<0.01). Corresponding result showed that, Axis 1 showed 30.71%variation. Effective number of codons (ENC) and GC3 were significantly correlated (p<0.01). The correlation between GC12 and GC3 were significant (p<0.01). 26 synonymous codons were the optimum codons which were all ended by C or G. The 3rd base composition preferred G/C in SS genes. Compared with cluster analysis dendrogram of RSCU for SS genes, the evolutionary tree build based on protein sequences encoded by SS genes through MEGA 5.0 software fit traditional viewpoint higher. The third position for all codons in SS preferred to end with A and T. Synonymous codon usage pattern formed under effect of mutation pressure and natural selection, mutation pressure influenced higher in SS gene.