CAJ | 학술논문

孤儿基因为某物种基因组内特有的,在其它物种内没有同源基因存在的特殊基因.对物种孤儿基因的鉴定和分析已成为比较基因组学研究的一个新兴领域.本研究通过生物信息学的分析,着重研究了49个葡萄孤儿基因在各发育阶段和各器官中的特异表达特性、亚细胞定位预测和共表达基因群的生物学过程基因本体论(GO)分析.结果显示,35个基因在NCBI的EST数据库中存在EST表达证据,其中有7个基因具有器官表达特异性,且大部分在生殖器官中特异表达.通过亚细胞定位预测到大部分葡萄孤儿基因为分泌蛋白.GO分析表明葡萄孤儿基因的共表达基因群主要参与寡肽转运和跨膜运输等生物学过程,这与葡萄孤儿基因的亚细胞定位预测结果相一致.本文研究结果将为葡萄孤儿基因的进一步功能分析提供重要研究基础.
고인기인위모물충기인조내특유적,재기타물충내몰유동원기인존재적특수기인.대물충고인기인적감정화분석이성위비교기인조학연구적일개신흥영역.본연구통과생물신식학적분석,착중연구료49개포도고인기인재각발육계단화각기관중적특이표체특성、아세포정위예측화공표체기인군적생물학과정기인본체론(GO)분석.결과현시,35개기인재NCBI적EST수거고중존재EST표체증거,기중유7개기인구유기관표체특이성,차대부분재생식기관중특이표체.통과아세포정위예측도대부분포도고인기인위분비단백.GO분석표명포도고인기인적공표체기인군주요삼여과태전운화과막운수등생물학과정,저여포도고인기인적아세포정위예측결과상일치.본문연구결과장위포도고인기인적진일보공능분석제공중요연구기출.
Orphan gene is a type of particular gene, which is unique in the genome of a certain species or has no known homologs in other species. The identification and characterization of orphan genes of a certain species has been an emerging field of comparative genomics. Using the approaches of bioinformatics, we mainly survey the development or organ specific expression, subcellular localization prediction and Gene Ontology (GO) analysis of 49 orphan genes in grape. We found that 35 grape orphan genes (GOGs) have EST evidence in NCBI EST database, and seven of them are organ specific expressed. Most of the organ-specific orphan genes are expressed after sexual maturation. Subcellular localization prediction revealed that the GOGs were significantly enriched in proteins targeted to the secretory pathway. GO analysis indicated the significant enrichment of the co-expressed genes of orphan genes in oligo-peptide transport and transmembrane transport functional categories, which are consistent w ith the subcellular localization prediction of GOGs. The results of this paper could provide a significant foundation for further research on the functional analysis of GOGs.