CAJ | 학술논문

cDNA-AFLP技术是近年来广泛应用于植物基因分离与表达研究的mRNA指纹图谱技术,在分离植物特异性基因等生物技术研究中发挥了巨大作用.在植物耐盐基因鉴定方面,该技术的应用处于新兴初始阶段,但已取得了诸多成效.本文在概述植物耐盐响应机制的基础上,着重对利用cDNA-AFLP技术鉴定的植物耐盐相关基因及其相应作用机制进行了阐述,并对其在植物耐盐分子生物学研究上的应用前景进行了展望.
cDNA-AFLP기술시근년래엄범응용우식물기인분리여표체연구적mRNA지문도보기술,재분리식물특이성기인등생물기술연구중발휘료거대작용.재식물내염기인감정방면,해기술적응용처우신흥초시계단,단이취득료제다성효.본문재개술식물내염향응궤제적기출상,착중대이용cDNA-AFLP기술감정적식물내염상관기인급기상응작용궤제진행료천술,병대기재식물내염분자생물학연구상적응용전경진행료전망.
cDNA-AFLP technique was a kind of mRNA fingerprint technology widely used in plant gene isolation and expression in recent years. By using the technique many achievements have been achieved in various fields especially in identification and isolation of specific genes. In plant salt-tolerance study this newly arisen technique was in the initial application stage and have gained some better early effects. On the basis of a brief description of mechanism of plant response to salt environment, the application of cDNA-AFLP technique in identification and isolation of salt-tolerance genes and their related action mechanisms were especially introduced. Finally, the prospect of cDNA-AFLP technique in molecular study of salt-inducible was further summarized.