中国农业科技导报
中國農業科技導報
중국농업과기도보
REVIEW OF CHINA AGRICULTURAL SCIENCE AND TECHNOLOGY
2013年
2期
83-88
,共6页
曾钢%叶艳英%曹鸣庆%马荣才%唐乐尘%姚磊
曾鋼%葉豔英%曹鳴慶%馬榮纔%唐樂塵%姚磊
증강%협염영%조명경%마영재%당악진%요뢰
芜菁花叶病毒%外壳蛋白基因%发卡结构%RNA干扰%抗性
蕪菁花葉病毒%外殼蛋白基因%髮卡結構%RNA榦擾%抗性
무정화협병독%외각단백기인%발잡결구%RNA간우%항성
turnip mosaic virus%coat protein gene%hairpin structure%RNAi%resistance
为了获得对芜菁花叶病毒(TuMV)高度稳定的抗性,选取TuMV的外壳蛋白(CP)基因近3′端保守区共377 bp的核苷酸序列,构建了抗TuMV的发卡结构RNAi载体,并转化了TuMV的天然宿主拟南芥.转基因植株用北京地区TuMV主要流行的强致病株系BJ-C4人工接种.鉴定的8个转基因株系中有3个株系表现出对TuMV的高度抗性.定量PCR结果显示,高抗转基因株系体内几乎检测不到病毒的积累.
為瞭穫得對蕪菁花葉病毒(TuMV)高度穩定的抗性,選取TuMV的外殼蛋白(CP)基因近3′耑保守區共377 bp的覈苷痠序列,構建瞭抗TuMV的髮卡結構RNAi載體,併轉化瞭TuMV的天然宿主擬南芥.轉基因植株用北京地區TuMV主要流行的彊緻病株繫BJ-C4人工接種.鑒定的8箇轉基因株繫中有3箇株繫錶現齣對TuMV的高度抗性.定量PCR結果顯示,高抗轉基因株繫體內幾乎檢測不到病毒的積纍.
위료획득대무정화협병독(TuMV)고도은정적항성,선취TuMV적외각단백(CP)기인근3′단보수구공377 bp적핵감산서렬,구건료항TuMV적발잡결구RNAi재체,병전화료TuMV적천연숙주의남개.전기인식주용북경지구TuMV주요류행적강치병주계BJ-C4인공접충.감정적8개전기인주계중유3개주계표현출대TuMV적고도항성.정량PCR결과현시,고항전기인주계체내궤호검측불도병독적적루.
In order to get stable high resistance to turnip mosaic virus (TuMV), a 377-nucleotide (nt) segment near 3′conserved region of TuMV coat protein (CP) gene was chosen to construct hairpin RNAi vector, and transformed into Arabidopsis thaliana. The transgenic plants were inoculated with TuMV isolate BJ-C4, which was of high pathogenicity and mainly epidemic in Beijing area. Three of the 8 transgenic lines showed hyper-resistance to TuMV. Quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants.
