CAJ | 학술논문

目的探讨脂肪肝与正常肝内VX2移植瘤模型的建立方法,旨在为临床脂肪肝肿瘤的研究提供依据.方法选用健康家兔34只,随机分为脂肪肝组(n=18)和正常对照组(n=16).脂肪肝组采用高脂饲料+酒精喂养,正常对照组采用基础饲料+自来水喂养,建立脂肪肝与正常肝内VX2移植瘤模型,应用常规超声及超声造影观察并与病理结果对照.结果⑴高脂饲料+酒精喂养法可成功建立兔脂肪肝模型,成模率100%.⑵开腹肝内组织块种植法可成功建立兔脂肪肝及正常肝内VX2移植瘤模型,成瘤率89.7%.⑶超声造影测量VX2移植瘤最大径与大体病理测值比较,无统计学差异(P>0.05).结论使用高脂饲料+酒精喂养可成功建立近似人类病理生理过程的脂肪肝模型.开腹组织块种植法建立兔VX2移植瘤模型方法简单,成瘤率高.
목적탐토지방간여정상간내VX2이식류모형적건립방법,지재위림상지방간종류적연구제공의거.방법선용건강가토34지,수궤분위지방간조(n=18)화정상대조조(n=16).지방간조채용고지사료+주정위양,정상대조조채용기출사료+자래수위양,건립지방간여정상간내VX2이식류모형,응용상규초성급초성조영관찰병여병리결과대조.결과⑴고지사료+주정위양법가성공건립토지방간모형,성모솔100%.⑵개복간내조직괴충식법가성공건립토지방간급정상간내VX2이식류모형,성류솔89.7%.⑶초성조영측량VX2이식류최대경여대체병리측치비교,무통계학차이(P>0.05).결론사용고지사료+주정위양가성공건립근사인류병리생리과정적지방간모형.개복조직괴충식법건립토VX2이식류모형방법간단,성류솔고.
Objective To explore establishing method of VX2 tumors in fatty 1iver and normal liver, Aimed at providing the basis for clinical fatty liver cancer research. Methods 34 rabbits were randomly divided into fatty 1iver group(n=18) and control group(n=16), Animals in fatty group were feeding high-fat diet and drinking water containing 5% ethanol, Animals in control group were feed regular diet and water. The model of fatty liver and normal liver VX2 tumor in rabbit were established, furthermore, compared with pathologic histology. Results ⑴Models of fatty 1iver disease could be established in rabbits by feeding them high-fat and alcohol drink, which the success rate was 100%. ⑵The successful rate of implantation VX2 tumor in fatty liver and normal liver of rabbit was 89.7%.⑶Measurements of tumor using contrast-enhanced ultrasound were consistent with pathology, showed no significant difference(P>0.05). Conclusion The animal model of fatty liver established by being fed a high-fat and alcohol diet was similar as human pathophysiological process fatty liver. The method implanting VX2 tumor lump to rabbit fatty liver was easy reproduction and high success rate.