CAJ | 학술논문

目的通过二血管阻断加低血压法建立大鼠全脑缺血再灌注模型,观察δ阿片受体激动剂DADLE对大鼠肺水转运的作用,探讨其对全脑缺血再灌注肺损伤(ALI)的保护作用.方法健康雌性SD大鼠30只随机分为假手术组(Sham)、模型组(I/R)、DADLE处理组各10只.假手术组(Sham):只暴露血管而不夹闭;缺血再灌注组(I/R):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15min后给予再灌注120min;DADLE处理组:于再灌注前经由左侧颈静注射DADLE 5mg/kg,再灌注120min后,取右肺组织,常规HE染色观察其形态学改变,左肺叶组织湿质量/干质量(W/D)值.并与假手术组(Sham)、模型组(I/R)进行对照分析.结果成模后的肺脏表现为肺泡间隔增宽,毛细血管扩张充血,肺胞腔内及血管周围中性粒细胞浸润,气管壁部分上皮脱落,肺胞腔及气管腔均有浆液渗出.DADLE处理组肺脏充血减轻,中性粒细胞浸润有所减少.结论大鼠急性全脑缺血再灌注模型对肺有不同程度的损伤,DADLE可减少肺组织液体过量分泌,对ALI具有一定的保护作用.
목적통과이혈관조단가저혈압법건립대서전뇌결혈재관주모형,관찰δ아편수체격동제DADLE대대서폐수전운적작용,탐토기대전뇌결혈재관주폐손상(ALI)적보호작용.방법건강자성SD대서30지수궤분위가수술조(Sham)、모형조(I/R)、DADLE처리조각10지.가수술조(Sham):지폭로혈관이불협폐;결혈재관주조(I/R):채용개량적이혈관조단가저혈압법건립전뇌결혈재관주모형,결혈15min후급여재관주120min;DADLE처리조:우재관주전경유좌측경정주사DADLE 5mg/kg,재관주120min후,취우폐조직,상규HE염색관찰기형태학개변,좌폐협조직습질량/간질량(W/D)치.병여가수술조(Sham)、모형조(I/R)진행대조분석.결과성모후적폐장표현위폐포간격증관,모세혈관확장충혈,폐포강내급혈관주위중성립세포침윤,기관벽부분상피탈락,폐포강급기관강균유장액삼출.DADLE처리조폐장충혈감경,중성립세포침윤유소감소.결론대서급성전뇌결혈재관주모형대폐유불동정도적손상,DADLE가감소폐조직액체과량분비,대ALI구유일정적보호작용.
Objective To occlusion combined with hypotension to establish the rat model of cerebral ischemia reperfusion by two vessels, observation of δ?opioid receptor agonist DADLE on the transport of water in the lung in rats, to explore its on cerebral ischemia reperfusion injury(ALI) and the protective effect of. Methods 30 healthy SD rats were randomly divided into sham operation group(Sham), model group(I/R), DADLE treatment group. Sham operation group(Sham, n=10): expose only blood vessels and no closed; ischemia reperfusion group(I/R, n=10): using improved two vessel occlusion of cerebral ischemia reperfusion model combined with hypotension, 15min after ischemia reperfusion was given 120min; DADLE treatment group(n=10) on reperfusion first through the left jugular venous injection of DADLE5mg/kg, 120min after reperfusion, and the right lung tissue, morphological changes were observed by routine HE staining, left lung tissue wet weight/dry weight(W/D) value. Compared with the sham operation group(Sham), model group(I/R) were analyzed. Results After injection of the lung showed alveolar septum, capillary dilatation and congestion, alveolar cavity and perivascular infiltration of neutrophils, tracheal wall portion of epithelial cell, lung and tracheal cavity are serous fluid exudation. DADLE treatment group reduced lung congestion, neutrophil infiltration decreased. Conclusion Acute cerebral ischemia reperfusion rat models with different degrees of damage to the lungs, DADLE can reduce lung fluid excess secretion, has a protective effect on ALI.