CAJ | 학술논문

目的确定接种量对重组Pichia pastoris GS115Muts菌株的影响和更适合目的菌株的培养基.方法采用三种不同的培养基(YPD培养基、BMGY培养基和复合培养基)培养重组Pichia pastoris GS115Muts菌株,以1.0%、1.5%、2.0%的接种量接种,分别在4h和24h的时间间隔下,采用比浊法对酵母菌96h内的生长进行OD值测定.结果复合培养基和YPD培养基是三种培养基较为合适的,且在12-24h之间是重组酵母菌的对数生长期,此时是接种的最佳时间,接种量对菌株的生长没有明显影响.结论合适的培养基和适当的接种时间对巴斯德毕赤酵母(Pichia pastoris)的培养关系密切.
목적학정접충량대중조Pichia pastoris GS115Muts균주적영향화경괄합목적균주적배양기.방법채용삼충불동적배양기(YPD배양기、BMGY배양기화복합배양기)배양중조Pichia pastoris GS115Muts균주,이1.0%、1.5%、2.0%적접충량접충,분별재4h화24h적시간간격하,채용비탁법대효모균96h내적생장진행OD치측정.결과복합배양기화YPD배양기시삼충배양기교위합괄적,차재12-24h지간시중조효모균적대수생장기,차시시접충적최가시간,접충량대균주적생장몰유명현영향.결론합괄적배양기화괄당적접충시간대파사덕필적효모(Pichia pastoris)적배양관계밀절.
Objectice This Experiment is testing the most suitable culture medium and the right time when the seed liquid is most applicable. Methods There are three kinds of culture medium in this experiment-YPD, BMGY, and compound culture medium. They are used to reform a new strain named Pichia pastoris GS115Muts. The OD is tested every 4 and 24 hours, within 96 hours, in the way of turbidimetric method. Result The compound culture medium and the YPD are the better culture medium in three mediums. And the best time to vaccinate is the period between 12 and 24 hours. Conclusion Pasteur Pichia yeast Strain is closely related to the Suitable medium and appropriate inoculation time.