CAJ | 학술논문

目的探讨三七总皂苷在体外对三种不同的人肝癌细胞株增殖的影响.方法体外培养人肝癌细胞株HepG2、BEL-7402、SMMC-7721和正常人肝细胞株 L-02,给予不同浓度(0、10、100、200、400和800μg·mL-1)的三七总皂苷分别培养24、48和72 h 后,采用 MTT 比色法检测细胞增殖的情况.结果不同浓度三七总皂苷处理正常肝细胞 L-02相同时间或相同浓度(800μg·mL-1)三七总皂苷处理正常肝细胞 L-02不同的时间时,其对细胞增殖的抑制率均较低.而不同浓度三七总皂苷处理 HepG2、BEL-7402和 SMMC-7721肝癌细胞株相同时间或相同浓度(800μg·mL-1)三七总皂苷处理不同的时间时,其细胞增殖抑制效应随作用浓度的增加或作用时间的延长而升高.结论三七总皂苷可显著抑制体外培养的三种不同人肝癌细胞株的增殖,且呈浓度与时间依赖性.
목적탐토삼칠총조감재체외대삼충불동적인간암세포주증식적영향.방법체외배양인간암세포주HepG2、BEL-7402、SMMC-7721화정상인간세포주 L-02,급여불동농도(0、10、100、200、400화800μg·mL-1)적삼칠총조감분별배양24、48화72 h 후,채용 MTT 비색법검측세포증식적정황.결과불동농도삼칠총조감처리정상간세포 L-02상동시간혹상동농도(800μg·mL-1)삼칠총조감처리정상간세포 L-02불동적시간시,기대세포증식적억제솔균교저.이불동농도삼칠총조감처리 HepG2、BEL-7402화 SMMC-7721간암세포주상동시간혹상동농도(800μg·mL-1)삼칠총조감처리불동적시간시,기세포증식억제효응수작용농도적증가혹작용시간적연장이승고.결론삼칠총조감가현저억제체외배양적삼충불동인간암세포주적증식,차정농도여시간의뢰성.
@@@@Objective To study the effects of Panax Notoginseng Saponins on the proliferation of three different kinds of human hepatoma cell lines in vitro. Methods Human hepatoma cell lines HepG2, BEL-7402, SMMC-7721 and normal liver cell line L-02 were cultured in different concentrations (0, 10, 100, 200, 400 and 800 μg·mL-1 ) of Panax Notoginseng Saponins for respectively 24, 48 and 72 h. Then the cellular proliferation inhibition rates were detected by MTT method. Results All the cellular proliferation inhibition rates of L-02 cell were low when treated by different concentrations of Panax Notoginseng Saponins for the same time or by the same concentration of Panax Notoginseng Saponins for different time. While the cellular proliferation inhibition rates of HepG2, BEL-7402 and SMMC-7721 cells treated by different concentra-tions of Panax Notoginseng Saponins for equal time or equal concentration (800 μg·ml-1) of Panax Notoginseng Saponins for different time were enhanced with the increase of working concentration or the extend of working time of Panax Noto-ginseng Saponins. Conclusion Panax Notoginseng Saponins could inhibit the proliferation of three different kinds of human hepatoma cell lines in vitro in a concentration and time dependent manner.