CAJ | 학술논문

神经干细胞(neural stem cel s, NSCs)移植治疗神经损伤被认为是具有潜在应用价值的手段,但其来源困难;骨髓间充质干细胞(bone marrow mesenchymal stem cel s, BMSCs)以其所具有的诸多优点,为神经损伤的治疗提供了一个新的思路.而 BMSCs 是否是通过作用于内源性的 NSCs 来促进神经修复,仍存在着争议.今采用海藻酸钙胶珠将NSCs 包囊培养至一定大小的神经球后,再与 BMSCs 进行共培养,考察 BMSCs 对生长在海藻酸钙胶珠内的 NSCs增殖与分化的作用,探讨 BMSCs 移植治疗神经疾病与损伤的作用机理.共培养过程中观察神经球结构的变化;共培养结束后计算 NSCs 的增殖倍数,对增殖条件下共培养的 NSCs 表型和多向分化潜能进行免疫荧光染色鉴定;对分化条件下共培养的 NSCs 向不同神经细胞分化的能力进行流式细胞仪检测.结果表明,BMSCs 可使生长于支架内的NSCs迁出细胞球,对 NSCs 的增殖没有明显影响;但能够明显影响 NSCs 的分化,使其向少突胶质细胞分化的能力增加3倍,向星形胶质细胞分化的能力减弱1倍,而向神经元细胞分化的能力没有明显变化.BMSCs 有可能是通过分泌某些因子增加了 NSCs 迁移及向少突胶质细胞分化的能力,从而促进神经损伤的修复.
신경간세포(neural stem cel s, NSCs)이식치료신경손상피인위시구유잠재응용개치적수단,단기래원곤난;골수간충질간세포(bone marrow mesenchymal stem cel s, BMSCs)이기소구유적제다우점,위신경손상적치료제공료일개신적사로.이 BMSCs 시부시통과작용우내원성적 NSCs 래촉진신경수복,잉존재착쟁의.금채용해조산개효주장NSCs 포낭배양지일정대소적신경구후,재여 BMSCs 진행공배양,고찰 BMSCs 대생장재해조산개효주내적 NSCs증식여분화적작용,탐토 BMSCs 이식치료신경질병여손상적작용궤리.공배양과정중관찰신경구결구적변화;공배양결속후계산 NSCs 적증식배수,대증식조건하공배양적 NSCs 표형화다향분화잠능진행면역형광염색감정;대분화조건하공배양적 NSCs 향불동신경세포분화적능력진행류식세포의검측.결과표명,BMSCs 가사생장우지가내적NSCs천출세포구,대 NSCs 적증식몰유명현영향;단능구명현영향 NSCs 적분화,사기향소돌효질세포분화적능력증가3배,향성형효질세포분화적능력감약1배,이향신경원세포분화적능력몰유명현변화.BMSCs 유가능시통과분비모사인자증가료 NSCs 천이급향소돌효질세포분화적능력,종이촉진신경손상적수복.
Neural stem cells (NSCs) transplantation is believed to be effective in the treatment of nerve injuries;however, they are limited and difficult to be isolated from brain. The bone marrow mesenchymal stem cells (BMSCs) possessing some advantages can be used as a new cell source for repairing nerve injuries. However, it is still not clear that isn’t the BMSCs effect on NSCs is the mechanism of promoting nerve repair. Therefore, in our research, the neurospheres of NSCs encapsulated in calcium alginate beads (Ca-Alg-Bs) were cocultured with BMSCs to investigate the effects of BMSCs on the proliferation and differentiation of NSCs growing in Ca-Alg-Bs and to explore the mechanism of recover neural diseases by BMSCs transplantation. During the coculture period, the neurosphere structure was observed, and when the coculture ended, the NSC expansion fold was measured; the phenotype and multi-differentiation potentials of NSCs in the proliferation medium were assayed by immunofluorescent staining. At the same time the differentiating capacity of NSCs into neurocytes in the differentiation medium was analyzed with flow cytometry. The results show that the NSCs in beads could migrate out of the neurospheres by contacting with BMSCs indirectly, and the BMSCs effect NSCs proliferation slightly, while influence NSCs differentiation obviously;so that the oligodendrocyte proportion increases 3 times and the astrocyte proportion decreases 1 fold, but the neuron proportion remains unchanged. The BMSCs probably secret some factors to encourage NSCs to migrate and differentiate into oligodendrocytes and subsequently accelerate the nerve recovery.