CAJ | 학술논문

目的:观察子午流注针法配合常规针刺对急性胃黏膜损伤家兔胃黏膜的修复作用,为临床治疗本病提供部分实验依据.方法:将40只雌雄各半的家兔随机分为5组,每组8只:空白组(A组)、模型组(B组)、常规针刺组(C组)、子午流注戌时取穴结合常规针刺组(D组)、子午流注辰时取穴结合常规针刺组(E组).采用无水乙醇造模法将家兔造成急性胃黏膜损伤模型,治疗7 d后剖腹取胃观察胃黏膜损伤指数(UI),检测胃黏膜组织中MT、HSP70含量的变化.结果:模型组UI值明显高于空白组(<0.01);与模型组比较,常规针刺组、戌时组、辰时组的UI值均有明显降低(<0.01);辰时组UI值低于常规针刺组、戌时组(<0.05);空白组的MT、HSP70含量均低于其它各组(<0.01);与模型组比较,常规针刺组、戌时组、辰时组的MT、HSP70含量均明显增高((<0.01);辰时组与常规针刺组、戌时组比较,MT、HSP70均含量增高(<0.05).结论:子午流注结合常规针刺针法能降低急性胃黏膜损伤家兔胃黏膜损伤指数(UI),对胃黏膜损伤后的修复起到积极的促进作用;子午流注结合常规针刺针法能促进胃黏膜MT、HSP70的合成与分泌,说明该治疗方法对胃黏膜的修复作用可能与MT,HSP70的作用机制有内在联系.
목적:관찰자오류주침법배합상규침자대급성위점막손상가토위점막적수복작용,위림상치료본병제공부분실험의거.방법:장40지자웅각반적가토수궤분위5조,매조8지:공백조(A조)、모형조(B조)、상규침자조(C조)、자오류주술시취혈결합상규침자조(D조)、자오류주신시취혈결합상규침자조(E조).채용무수을순조모법장가토조성급성위점막손상모형,치료7 d후부복취위관찰위점막손상지수(UI),검측위점막조직중MT、HSP70함량적변화.결과:모형조UI치명현고우공백조(<0.01);여모형조비교,상규침자조、술시조、신시조적UI치균유명현강저(<0.01);신시조UI치저우상규침자조、술시조(<0.05);공백조적MT、HSP70함량균저우기타각조(<0.01);여모형조비교,상규침자조、술시조、신시조적MT、HSP70함량균명현증고((<0.01);신시조여상규침자조、술시조비교,MT、HSP70균함량증고(<0.05).결론:자오류주결합상규침자침법능강저급성위점막손상가토위점막손상지수(UI),대위점막손상후적수복기도적겁적촉진작용;자오류주결합상규침자침법능촉진위점막MT、HSP70적합성여분비,설명해치료방법대위점막적수복작용가능여MT,HSP70적작용궤제유내재련계.
Objective: To observe the effect of repair on gastric mucosa injury in rabbit by midday-midnight point selec-tion combined with routine acupuncture, in order to offer some experimental evidence for treatment of this disease. Methods:40 rabbits were divided randomly into 5 groups, 8 in each group, including blank group (group A), model group (group B), routine acupuncture group (group C), midday-midnight point wushi selection combined with routine acupuncture group (group D) and midday-midnight point chenshi selection combined with routine acupuncture group (group E). Absolute alcohol model-ing was applied to establish the model of gastric mucosa injury in rabbit, and the ulcer index (UI), MT and HSP70 were ob-served after the treatment for 7 days. Results: The UI in the model group was higher than that of the blank group( <0.01);compared to the model group, the UI of the routine acupuncture group, wushi group and chenshi group were lower than that of the model group( <0.01); the UI of the chenshi group was lower than that of the routine acupuncture group and wushi group( <0.05); the MT and HSP70 in the blank group were lower than that of the other groups( <0.01); compared to the model group, the MT and HSP70 of the routine acupuncture group, wushi group and chenshi group were higher than that of the model group( <0.01); the MT and HSP70 of the chenshi group were higher than that of the routine acupuncture group and wushi group( <0.05). Conclusion: Midday-midnight point selection combined with routine acupuncture can reduce the UI in the model of gastric mucosa injury in rabbit, provide positive effect on repair of the gastric mucosa injury and promote the production of MT and HSP70 of gastric mucosa. The repair effect of this treatment might be related to the influence on MT and HSP70.